Table 1. Quantitation of absolute... | American Society of Hematology

Table 1.

Quantitation of absolute numbers of EBV-specific and alloreactive T cells

	Day 0	Day 7	Day 14	Day 21	Day 28
Donor A
Intracellular IFN-γ production^*
Anti-EBV CD3⁺	700	3 100	15 600	13 500	23 100
Anti-allo CD3⁺	1 100	500	600	400	200
Anti-EBV CD3⁺CD8⁺	315	1 880	11 407	9 928	16 660
Anti-allo CD3⁺CD8⁺	495	564	366	204	136
Anti-EBV CD3⁺CD4⁺	80	810	2 520	4 536	5 848
Anti-allo CD3⁺CD4⁺	ND	54	42	72	170
Limiting-dilution analysis^†
Anti-EBV CD3⁺	28	35	274	300	333
Anti-allo CD3⁺	36	6.6	4.1	1.4	0.9
Donor B
Intracellular IFN-γ production^*
Anti-EBV CD3⁺	900	1 000	5 100	6 700	5 200
Anti-allo CD3⁺	600	400	200	100	ND
Anti-EBV CD3⁺CD8⁺	448	580	2 117	6 298	7 200
Anti-allo CD3⁺CD8⁺	288	435	232	67	ND
Anti-EBV CD3⁺CD4⁺	657	420	2 982	1 800	561
Anti-allo CD3⁺CD4⁺	365	70	71	ND	ND
Limiting-dilution analysis^†
Anti-EBV CD3⁺	10	22	38	70	132
Anti-allo CD3⁺	8	23	3	1	0.8

	Day 0	Day 7	Day 14	Day 21	Day 28
Donor A
Intracellular IFN-γ production^*
Anti-EBV CD3⁺	700	3 100	15 600	13 500	23 100
Anti-allo CD3⁺	1 100	500	600	400	200
Anti-EBV CD3⁺CD8⁺	315	1 880	11 407	9 928	16 660
Anti-allo CD3⁺CD8⁺	495	564	366	204	136
Anti-EBV CD3⁺CD4⁺	80	810	2 520	4 536	5 848
Anti-allo CD3⁺CD4⁺	ND	54	42	72	170
Limiting-dilution analysis^†
Anti-EBV CD3⁺	28	35	274	300	333
Anti-allo CD3⁺	36	6.6	4.1	1.4	0.9
Donor B
Intracellular IFN-γ production^*
Anti-EBV CD3⁺	900	1 000	5 100	6 700	5 200
Anti-allo CD3⁺	600	400	200	100	ND
Anti-EBV CD3⁺CD8⁺	448	580	2 117	6 298	7 200
Anti-allo CD3⁺CD8⁺	288	435	232	67	ND
Anti-EBV CD3⁺CD4⁺	657	420	2 982	1 800	561
Anti-allo CD3⁺CD4⁺	365	70	71	ND	ND
Limiting-dilution analysis^†
Anti-EBV CD3⁺	10	22	38	70	132
Anti-allo CD3⁺	8	23	3	1	0.8

Absolute numbers were determined per 10⁵ T lymphocytes by LDA and intracellular IFN-γ production. EBV-specific and alloreactive CD3⁺ cells per 10⁵ T lymphocytes were determined by LDA at weekly incremental time points from day 0 to day 28 during the generation of EBV-specific T-cell lines of donors A and B. Results were compared to EBV-specific and alloreactive CD3⁺ cells producing intracellular IFN-γ per 10⁵ T lymphocytes. FACS analysis also determined the CD8⁺IFN-γ⁺ and CD4⁺IFN-γ⁺ subpopulations per 10⁵T cells.

ND indicates not detectable.

Absolute numbers of EBV-specific and alloreactive T cells per 10⁵ T lymphocytes by intracellular IFN-γ production.

†

Absolute numbers of EBV-specific and alloreactive T cells per 10⁵ T lymphocytes obtained by LDA.

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