Table 2. PCR and sequence analysis of V... | American Society of Hematology

Table 2.

PCR and sequence analysis of V gene rearrangements of single B cells from individual follicles of three follicular lymphomas

	Samples	V gene	No. of cells positive/cells analyzed		Clonal rearrangements^*
	Samples	V gene	Total	Per follicle	Clonal rearrangements^*
Case
1	FL cells	V_H	33/150	1:12/50	30 × VH3-23
				2:14/50	2 × VH3-23
				3:7/50	1 × VH3-11
	FL cells	V_κ	27/150	1:7/50	25 × VκB3
				2:12/50	2 × VκB3
				3:8/50
2	FL cells	V_H	71/150	1:30/50	71 × VH3-23
				2:23/50
				3:18/50
	FL cells	V_κ	55/150	1:20/50	55 × VκL2
				2:15/50
				3:20/50
3	FL cells	V_H	37/150	1:8/50	37 × VH3-49
				2:12/50
				3:17/49
	FL cells	V_λ	30/150	1:8/50	30 × Vλ3r
				2:10/50
				3:12/50
Controls^†
1	T cells	V_H	0/60
		V_κ	0/60
	buffer	V_H	0/60
		V_κ	0/60
2	T cells	V_H	3/60
		V_κ	4/60
	buffer	V_H	1/60
		V_κ	0/60
3	T cells	V_H	1/60
		V_λ	1/60
	buffer	V_H	1/60
		V_λ	1/60

	Samples	V gene	No. of cells positive/cells analyzed		Clonal rearrangements^*
	Samples	V gene	Total	Per follicle	Clonal rearrangements^*
Case
1	FL cells	V_H	33/150	1:12/50	30 × VH3-23
				2:14/50	2 × VH3-23
				3:7/50	1 × VH3-11
	FL cells	V_κ	27/150	1:7/50	25 × VκB3
				2:12/50	2 × VκB3
				3:8/50
2	FL cells	V_H	71/150	1:30/50	71 × VH3-23
				2:23/50
				3:18/50
	FL cells	V_κ	55/150	1:20/50	55 × VκL2
				2:15/50
				3:20/50
3	FL cells	V_H	37/150	1:8/50	37 × VH3-49
				2:12/50
				3:17/49
	FL cells	V_λ	30/150	1:8/50	30 × Vλ3r
				2:10/50
				3:12/50
Controls^†
1	T cells	V_H	0/60
		V_κ	0/60
	buffer	V_H	0/60
		V_κ	0/60
2	T cells	V_H	3/60
		V_κ	4/60
	buffer	V_H	1/60
		V_κ	0/60
3	T cells	V_H	1/60
		V_λ	1/60
	buffer	V_H	1/60
		V_λ	1/60

V genes unrelated to the tumor clones are shown in italics. There were 2 cells that harbored a VH3-23 and VκB3 rearrangement that were clonally related to each other.

†

Summary of controls with samples of buffer covering the sections. T cells were micromanipulated from adjacent tissue sections of the same cases as additional negative controls.

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