TaqMan real-time quantitative RT-PCR analysis of the expression of GSH2
| Sample . | CTvalue* . | ΔCT . | |
|---|---|---|---|
| GAPD . | GSH2 . | ||
| Fetal brain | 15.5 | 26.0 | 10.5 |
| Bone marrow | 19.6 ± 0.2 | Greater than 40 | No expression |
| Peripheral blood | 23.4 ± 0.2 | Greater than 40 | No expression |
| Spleen | 22.6 ± 0.3 | Greater than 40 | No expression |
| HL60 cell line | 16.0 ± 0.4 | Greater than 40 | No expression |
| CD34+ | 21.1 ± 0.9 | Greater than 40 | No expression |
| CD34+CD38− | 33.3 ± 0.6 | Greater than 40 | No expression |
| CD34+CD38+ | 24.5 ± 1.3 | Greater than 40 | No expression |
| CD34+CD7+ | 26.4 ± 0.2 | Greater than 40 | No expression |
| CD34+CD13+CD33+ | 23.2 ± 0.5 | Greater than 40 | No expression |
| CD34− | 18.0 ± 1.0 | Greater than 40 | No expression |
| CD34−CD3+ | 27.3 ± 0.6 | Greater than 40 | No expression |
| CD34−CD13+ | 30.3 ± 0.6 | Greater than 40 | No expression |
| CD34−CD33+ | 27.7 ± 1.2 | Greater than 40 | No expression |
| BM from case 1 | 22.7 | 25.4 | 3.3 ± 0.8 |
| BM from case 2 | 24.9 | 30.3 | 5.7 ± 0.3 |
| PB from case 3 | 25.0 | 33.8 | 9.6 ± 0.7 |
| BM from case 4 | 22.2 | 26.9 | 4.2 ± 0.7 |
| BM from case 5† | 28.3 | 37.1 | 9.0 ± 0.5 |
| BM from case 6 | Nd | nd | nd |
| Water‡ | Greater than 40 | Greater than 40 | No expression |
| Sample . | CTvalue* . | ΔCT . | |
|---|---|---|---|
| GAPD . | GSH2 . | ||
| Fetal brain | 15.5 | 26.0 | 10.5 |
| Bone marrow | 19.6 ± 0.2 | Greater than 40 | No expression |
| Peripheral blood | 23.4 ± 0.2 | Greater than 40 | No expression |
| Spleen | 22.6 ± 0.3 | Greater than 40 | No expression |
| HL60 cell line | 16.0 ± 0.4 | Greater than 40 | No expression |
| CD34+ | 21.1 ± 0.9 | Greater than 40 | No expression |
| CD34+CD38− | 33.3 ± 0.6 | Greater than 40 | No expression |
| CD34+CD38+ | 24.5 ± 1.3 | Greater than 40 | No expression |
| CD34+CD7+ | 26.4 ± 0.2 | Greater than 40 | No expression |
| CD34+CD13+CD33+ | 23.2 ± 0.5 | Greater than 40 | No expression |
| CD34− | 18.0 ± 1.0 | Greater than 40 | No expression |
| CD34−CD3+ | 27.3 ± 0.6 | Greater than 40 | No expression |
| CD34−CD13+ | 30.3 ± 0.6 | Greater than 40 | No expression |
| CD34−CD33+ | 27.7 ± 1.2 | Greater than 40 | No expression |
| BM from case 1 | 22.7 | 25.4 | 3.3 ± 0.8 |
| BM from case 2 | 24.9 | 30.3 | 5.7 ± 0.3 |
| PB from case 3 | 25.0 | 33.8 | 9.6 ± 0.7 |
| BM from case 4 | 22.2 | 26.9 | 4.2 ± 0.7 |
| BM from case 5† | 28.3 | 37.1 | 9.0 ± 0.5 |
| BM from case 6 | Nd | nd | nd |
| Water‡ | Greater than 40 | Greater than 40 | No expression |
The CT value was determined in at least 3 independent experiments. It is indicated as mean ± SD when no expression of GSH2 was detected. When expression ofGSH2 was detected, individual CT values are given from 1 representative experiment and the mean ± SD is determined from the ΔCT values obtained from the independent experiments.
RNA was extracted from fixed cells.
Water was used as “no template control” in each experiment.