Genes upregulated by doxycycline in K562-derived Gab2WT-6 and Gab2WT-7 cells
| Accession no. . | Name . | pSTAR-2 . | Gab2WT-6 . | Gab2WT-7 . | Average . |
|---|---|---|---|---|---|
| U25997 | Stanniocalcin precursor | ∼ 34.9 | ∼ 28.8 | ∼ 31.85 | |
| L27624 | Tissue factor pathway inhibitor-2 | 14.9 | 9.9 | 12.4 | |
| U70981 | IL-13 receptor | ∼ 10.4 | ∼ 13.8 | ∼ 12.1 | |
| M22612 | Pancreatic trypsin 1 | 2.7 | 16.1 | 9.4 | |
| X52022 | Type VI collagen alpha 3 chain | 4.2 | 13.5 | 8.85 | |
| M35999 | Platelet glycoprotein IIIa (CD61) | ∼ 8.7 | ∼ 8.5 | ∼ 8.6 | |
| X14787 | Thrombospondin | ∼ − 2.4 | 7.5 | ∼ 7.8 | ∼ 7.65 |
| D29992 | Placental protein 5 | 8.3 | 3.9 | 6.1 | |
| U07807 | Metallothionein IV | 6.1 | 3.8 | 4.95 | |
| M26901 | Renin | 4.3 | 5.4 | 4.85 | |
| L05424 | CD44 | ∼ 2.2 | 5.3 | 4 | 4.65* |
| U15932 | Dual-specificity protein phosphatase-5 | 5.7 | 3.4 | 4.55 | |
| X71345 | Trypsinogen IV, b-form | 2.7 | 6.3 | 4.5 | |
| J03040 | SPARC/osteonectin | 6.2 | 2.1 | 4.15 | |
| U81607 | Gravin (AKAP250) | 3.6 | 4.4 | 4 | |
| U03397 | Receptor protein 4-1BB (CD137) | 3.3 | 4.7 | 4 | |
| HG2981-HT3125† | Epican, alt splice 1 | 3.4 | 4.3 | 3.85 | |
| U16799 | Na, K ATPase beta-1 subunit | 3.5 | 4.1 | 3.8 | |
| AF014958 | Chemokine receptor X | 3.9 | 3.6 | 3.75 | |
| HG4069-HT4339† | Monocyte chemotactic protein 1 | 4.2 | 3.2 | 3.7 | |
| HG721-HT4827† | Placental protein 14, alt splice 2 | 4.1 | 3.1 | 3.6 | |
| HG2981-HT3127† | Epican, alt splice 11 | 3.1 | 3.8 | 3.45 | |
| M55998 | α1 collagen type I gene | 4.2 | 2.3 | 3.25 | |
| X04412 | Gelsolin | 3.3 | 3.1 | 3.2 | |
| U31384 | G protein γ-11 subunit | 3.6 | 2.5 | 3.05 | |
| L19058 | Glutamate receptor (GLUR5) | 3.1 | 2.8 | 2.95 | |
| D29642 | KIAA0053 | 2.2 | 3.3 | 2.75 | |
| M34344 | Platelet glycoprotein IIb (CD41) | 2.2 | 3.2 | 2.7 | |
| U34879 | 17-beta-hydroxysteroid dehydrogenase | − 2.4 | 2.9 | 2.4 | 2.65 |
| Z30426 | CD69 | 3.3 | 2 | 2.65 | |
| S45630 | Alpha B-crystallin | 2.7 | 2.5 | 2.6 | |
| HG721-HT4828† | Placental protein 14, alt splice 3 | 3 | 2.2 | 2.6 | |
| L13943 | Glycerol kinase | 2.1 | 2.5 | 2.6 | 2.55* |
| AF001294 | IPL (Tssc3) | 2 | 3.1 | 2.55 | |
| Z22534 | ALK-2 | 2.2 | 2.4 | 2.3 | |
| J03764 | Plasminogen activator inhibitor-1 | 2.1 | 2.5 | 2.3 | |
| AF010193 | SMAD7 | ∼ − 54.5 | 2.2 | 2.2 | 2.2 |
| HG2788-HT2896† | Calcyclin | 2 | 2.1 | 2.05 |
| Accession no. . | Name . | pSTAR-2 . | Gab2WT-6 . | Gab2WT-7 . | Average . |
|---|---|---|---|---|---|
| U25997 | Stanniocalcin precursor | ∼ 34.9 | ∼ 28.8 | ∼ 31.85 | |
| L27624 | Tissue factor pathway inhibitor-2 | 14.9 | 9.9 | 12.4 | |
| U70981 | IL-13 receptor | ∼ 10.4 | ∼ 13.8 | ∼ 12.1 | |
| M22612 | Pancreatic trypsin 1 | 2.7 | 16.1 | 9.4 | |
| X52022 | Type VI collagen alpha 3 chain | 4.2 | 13.5 | 8.85 | |
| M35999 | Platelet glycoprotein IIIa (CD61) | ∼ 8.7 | ∼ 8.5 | ∼ 8.6 | |
| X14787 | Thrombospondin | ∼ − 2.4 | 7.5 | ∼ 7.8 | ∼ 7.65 |
| D29992 | Placental protein 5 | 8.3 | 3.9 | 6.1 | |
| U07807 | Metallothionein IV | 6.1 | 3.8 | 4.95 | |
| M26901 | Renin | 4.3 | 5.4 | 4.85 | |
| L05424 | CD44 | ∼ 2.2 | 5.3 | 4 | 4.65* |
| U15932 | Dual-specificity protein phosphatase-5 | 5.7 | 3.4 | 4.55 | |
| X71345 | Trypsinogen IV, b-form | 2.7 | 6.3 | 4.5 | |
| J03040 | SPARC/osteonectin | 6.2 | 2.1 | 4.15 | |
| U81607 | Gravin (AKAP250) | 3.6 | 4.4 | 4 | |
| U03397 | Receptor protein 4-1BB (CD137) | 3.3 | 4.7 | 4 | |
| HG2981-HT3125† | Epican, alt splice 1 | 3.4 | 4.3 | 3.85 | |
| U16799 | Na, K ATPase beta-1 subunit | 3.5 | 4.1 | 3.8 | |
| AF014958 | Chemokine receptor X | 3.9 | 3.6 | 3.75 | |
| HG4069-HT4339† | Monocyte chemotactic protein 1 | 4.2 | 3.2 | 3.7 | |
| HG721-HT4827† | Placental protein 14, alt splice 2 | 4.1 | 3.1 | 3.6 | |
| HG2981-HT3127† | Epican, alt splice 11 | 3.1 | 3.8 | 3.45 | |
| M55998 | α1 collagen type I gene | 4.2 | 2.3 | 3.25 | |
| X04412 | Gelsolin | 3.3 | 3.1 | 3.2 | |
| U31384 | G protein γ-11 subunit | 3.6 | 2.5 | 3.05 | |
| L19058 | Glutamate receptor (GLUR5) | 3.1 | 2.8 | 2.95 | |
| D29642 | KIAA0053 | 2.2 | 3.3 | 2.75 | |
| M34344 | Platelet glycoprotein IIb (CD41) | 2.2 | 3.2 | 2.7 | |
| U34879 | 17-beta-hydroxysteroid dehydrogenase | − 2.4 | 2.9 | 2.4 | 2.65 |
| Z30426 | CD69 | 3.3 | 2 | 2.65 | |
| S45630 | Alpha B-crystallin | 2.7 | 2.5 | 2.6 | |
| HG721-HT4828† | Placental protein 14, alt splice 3 | 3 | 2.2 | 2.6 | |
| L13943 | Glycerol kinase | 2.1 | 2.5 | 2.6 | 2.55* |
| AF001294 | IPL (Tssc3) | 2 | 3.1 | 2.55 | |
| Z22534 | ALK-2 | 2.2 | 2.4 | 2.3 | |
| J03764 | Plasminogen activator inhibitor-1 | 2.1 | 2.5 | 2.3 | |
| AF010193 | SMAD7 | ∼ − 54.5 | 2.2 | 2.2 | 2.2 |
| HG2788-HT2896† | Calcyclin | 2 | 2.1 | 2.05 |
Data show the -fold increase. Listed here are genes that showed at least 2-fold changes in average signal intensity in both K562/Gab2WT-6 and K562/Gab2WT-7 cells, and changes of the corresponding genes in K562/pSTAR-2 cells. No gene was found to be downregulated greater than 2-fold in both K562/Gab2WT-6 and K562/Gab2WT-7 cells by dox induction. Inspection of raw intensity data was used to confirm signal intensity changes.
WT indicates wildtype; ∼, approximation; IL, interleukin.
Greater than 2-fold increase was detected in the control K562/pSTAR-2 cells.
Human Genome sequence number.