T-cell clones used in the study
| Clone . | Phenotype . | IFN-γ (pg/mL) . | IL-4 (pg/mL) . | T-cell type . |
|---|---|---|---|---|
| AL 1.16 | CD4+ | 1812 ± 109 | 15 ± 2 | Th1 |
| CS 2.43 | CD4+ | 2234 ± 102 | 13 ± 4 | Th1 |
| EF 1.3 | CD4+ | 4031 ± 156 | 130 ± 21 | Th1 |
| FA 1.42 | CD8+ | 2078 ± 167 | 52 ± 9 | Tc1 |
| AL 1.19 | CD8+ | 1974 ± 172 | 68 ± 7 | Tc1 |
| MB 2.9 | CD8+ | 2343 ± 188 | 15 ± 4 | Tc1 |
| AL 1.15 | CD4+ | ND | 3105 ± 265 | Th2 |
| CS 2.3 | CD4+ | 151 ± 23 | 6775 ± 436 | Th2 |
| EF 2.4 | CD4+ | ND | 6540 ± 541 | Th2 |
| FA 2.26 | CD8+ | ND | 7462 ± 854 | Tc2 |
| CS 2.12 | CD8+ | 51 ± 4 | 2437 ± 484 | Tc2 |
| MB 2.27 | CD8+ | 27 ± 4 | 1585 ± 123 | Tc2 |
| Clone . | Phenotype . | IFN-γ (pg/mL) . | IL-4 (pg/mL) . | T-cell type . |
|---|---|---|---|---|
| AL 1.16 | CD4+ | 1812 ± 109 | 15 ± 2 | Th1 |
| CS 2.43 | CD4+ | 2234 ± 102 | 13 ± 4 | Th1 |
| EF 1.3 | CD4+ | 4031 ± 156 | 130 ± 21 | Th1 |
| FA 1.42 | CD8+ | 2078 ± 167 | 52 ± 9 | Tc1 |
| AL 1.19 | CD8+ | 1974 ± 172 | 68 ± 7 | Tc1 |
| MB 2.9 | CD8+ | 2343 ± 188 | 15 ± 4 | Tc1 |
| AL 1.15 | CD4+ | ND | 3105 ± 265 | Th2 |
| CS 2.3 | CD4+ | 151 ± 23 | 6775 ± 436 | Th2 |
| EF 2.4 | CD4+ | ND | 6540 ± 541 | Th2 |
| FA 2.26 | CD8+ | ND | 7462 ± 854 | Tc2 |
| CS 2.12 | CD8+ | 51 ± 4 | 2437 ± 484 | Tc2 |
| MB 2.27 | CD8+ | 27 ± 4 | 1585 ± 123 | Tc2 |
Resting T-cell clones were stimulated with plate-bound anti-CD3 and soluble anti-CD28 monoclonal antibodies. After 48 hours, cytokine concentrations in the supernatants were measured by enzyme-linked immunosorbent assay. Unstimulated T cells did not produce detectable amounts of cytokines.
IFN-γ indicates interferon γ; IL-4, interleukin 4; Th, T helper; Tc, T cytotoxic; and ND, not detectable.