Effects of ATP, UTP, and LPS on release of 4 chemokines from dendritic cells
| Treatment . | IP-10 (ng/mL) . | RANTES (ng/mL) . | MDC (ng/mL) . | TARC (ng/mL) . |
|---|---|---|---|---|
| None | ND | ND | 65.2 ± 4.9 | 122 ± 16.2 |
| ATP | ND | ND | 165 ± 15.2* | 172 ± 28.3 |
| UTP | ND | ND | 72.1 ± 9.0 | 134 ± 12.7 |
| LPS | 11.3 ± 1.2 | 18.1 ± 1.7 | 315 ± 32.4 | 283 ± 27.1 |
| LPS + ATP | ND | 4.3 ± 0.6† | 512 ± 37.2† | 316 ± 29.6 |
| LPS + UTP | 10.8 ± 0.8 | 17.5 ± 1.9 | 307 ± 35.4 | 266 ± 21.9 |
| Treatment . | IP-10 (ng/mL) . | RANTES (ng/mL) . | MDC (ng/mL) . | TARC (ng/mL) . |
|---|---|---|---|---|
| None | ND | ND | 65.2 ± 4.9 | 122 ± 16.2 |
| ATP | ND | ND | 165 ± 15.2* | 172 ± 28.3 |
| UTP | ND | ND | 72.1 ± 9.0 | 134 ± 12.7 |
| LPS | 11.3 ± 1.2 | 18.1 ± 1.7 | 315 ± 32.4 | 283 ± 27.1 |
| LPS + ATP | ND | 4.3 ± 0.6† | 512 ± 37.2† | 316 ± 29.6 |
| LPS + UTP | 10.8 ± 0.8 | 17.5 ± 1.9 | 307 ± 35.4 | 266 ± 21.9 |
Dendritic cells were left untreated or stimulated with either 250 μM ATP, 250 μM UTP, 10 μg/mL LPS, LPS and ATP, or LPS and UTP. After 24 hours, chemokine levels in the supernatants were measured by enzyme-linked immunosorbent assay. Results are mean ± SD nanograms per milliliter/106 cells from 5 independent experiments.
ATP indicates adenosine triphosphate; UTP, uridine triphosphate; LPS, lipopolysaccharide; IP-10, interferon-inducible protein 10; RANTES, regulated upon activation, normal T-cell expressed and secreted; MDC, macrophage-derived chemokine; TARC, thymus- and activation-regulated chemokine; and ND, not detectable.
P < .03 compared with results with untreated or UTP-treated DCs.
P < .03 compared with results with DCs treated with LPS alone or with LPS and UTP.