Cytokine production after stimulation of human T cells from spleen and thymus
| Experiment . | IL-2 (pg/mL) . | IL-4 (pg/mL) . | IFN-γ (pg/mL) . |
|---|---|---|---|
| 1 | 44.5 | 3.0 | 316.5 |
| 2 | 13.3 | 21.9 | 289.0 |
| 3 | 157.5 | 13.5 | 424.0 |
| Experiment . | IL-2 (pg/mL) . | IL-4 (pg/mL) . | IFN-γ (pg/mL) . |
|---|---|---|---|
| 1 | 44.5 | 3.0 | 316.5 |
| 2 | 13.3 | 21.9 | 289.0 |
| 3 | 157.5 | 13.5 | 424.0 |
Human cells were cultured from murine spleen by stimulation with PHA. After washing, these cells were stimulated with anti-CD3 + TPA or ConA. Supernatants were harvested 24 hours later, and cytokine content was measured. Data shown are from 3 mice injected from the same UCB donor, and numbers shown are averages from 2 stimulation methods.