Table 1. PCR primers with corresponding... | American Society of Hematology

Table 1.

PCR primers with corresponding annealing temperatures

Primer	5′-3′ Primer sequence	Annealing temperature, °C
Human dCK
Forward
A7	TCT TTG CCG GAC GAG CTC TG	65
T5-BamHI	GGA AGG ATC CAC CAT GGC CAC CCC GCC CAA	70
A6	TAA GGA ATG GCC ACC CCG CC	55
Reverse
B5	TGG AAC CAT TTG GCT GCC TG	65
B6-BamHI	CGC TGG ATC CAA GAT CAC AAA GTA CTC AA	70
B6	CAA GAT CAC AAA GTA CTC AA	55
Rat dCK
Forward
A10	ATG GCC ACC CCA CCT AAG AGG TT	65
T7	GGA TCC TAA TAC GAC TCA CTA TAG GAA CAG ACC ACC ATG GCC ACC CCA CCT AAG AGG	55
Reverse
B5	TTG CCT GTT GTC TCC TGT GC	65
B6	TGC AAT CAC AAA GTA CTC AA	55

Primer	5′-3′ Primer sequence	Annealing temperature, °C
Human dCK
Forward
A7	TCT TTG CCG GAC GAG CTC TG	65
T5-BamHI	GGA AGG ATC CAC CAT GGC CAC CCC GCC CAA	70
A6	TAA GGA ATG GCC ACC CCG CC	55
Reverse
B5	TGG AAC CAT TTG GCT GCC TG	65
B6-BamHI	CGC TGG ATC CAA GAT CAC AAA GTA CTC AA	70
B6	CAA GAT CAC AAA GTA CTC AA	55
Rat dCK
Forward
A10	ATG GCC ACC CCA CCT AAG AGG TT	65
T7	GGA TCC TAA TAC GAC TCA CTA TAG GAA CAG ACC ACC ATG GCC ACC CCA CCT AAG AGG	55
Reverse
B5	TTG CCT GTT GTC TCC TGT GC	65
B6	TGC AAT CAC AAA GTA CTC AA	55