Tumor-infiltrating inflammatory cells and tumor MVD
| Treatment . | CD19 . | CD4 . | CD8 . | CD11b . | NK1.1 . | IFN−γ . | MVD . |
|---|---|---|---|---|---|---|---|
| Saline | 0 | 0.37 ± 0.17 | 0.55 ± 0.27 | 11.56 ± 2.55 | 10.63 ± 9.01 | 8.10 ± 2.19 | 39.65 ± 3.52 |
| IL-2/L19 mix | 0 | 0.74 ± 0.24 | 0.60 ± 0.35 | 18.92 ± 4.19 | 5.58 ± 2.86 | 4.29 ± 1.51 | 38.1 ± 1.90 |
| L19–IL-2 | 0 | 2.13 ± 0.50 | 9.75 ± 6.45 | 38.16 ± 7.42 | 26.42 ± 6.38 | 29.34 ± 8.04 | 33.83 ± 2.97 |
| Treatment . | CD19 . | CD4 . | CD8 . | CD11b . | NK1.1 . | IFN−γ . | MVD . |
|---|---|---|---|---|---|---|---|
| Saline | 0 | 0.37 ± 0.17 | 0.55 ± 0.27 | 11.56 ± 2.55 | 10.63 ± 9.01 | 8.10 ± 2.19 | 39.65 ± 3.52 |
| IL-2/L19 mix | 0 | 0.74 ± 0.24 | 0.60 ± 0.35 | 18.92 ± 4.19 | 5.58 ± 2.86 | 4.29 ± 1.51 | 38.1 ± 1.90 |
| L19–IL-2 | 0 | 2.13 ± 0.50 | 9.75 ± 6.45 | 38.16 ± 7.42 | 26.42 ± 6.38 | 29.34 ± 8.04 | 33.83 ± 2.97 |
A mouse colon adenocarcinoma cell line (C51) was subcutaneously implanted in syngeneic mice. Three days after implantation, animals were treated with L19–IL-2 fusion protein or IL-2/L19 mix (72 000 IU IL-2) or saline by daily injection into the tail vein for 6 days. Tumor sections from the 3 different groups were immunohistochemically analyzed with a panel of antibodies. Values represent stained area as % of the measurement frame area (500 × 370 μm), ± SE (see “Materials and methods”). The differences in the levels of CD8, NK, and IFN-γ among tumors from animals treated with the fusion protein L19–IL-2 and an equimolar mixture of IL-2/L19 were statistically highly significant (P < .02). On the contrary, no statistically significant differences were observed in any of the tested antigens in tumors from animals treated with the mixture IL-2/L19 or saline alone.