Histologic analysis of F9 tumor growth in syngeneic mice treated with L19–IL-2 and D1.3–IL-2 fusion proteins
| . | Treatment . | ||
|---|---|---|---|
| L19–IL2 . | D1.3–IL2 . | Saline . | |
| Weight of tumors (g) | 0.05 ± 0.01 | 0.597 ± 0.1 | 0.811 ± 0.23 |
| TILs* | 53.2 ± 13.8 | 0.75 ± 0.14 | 0.52 ± 0.26 |
| M/HPF | 5.2 ± 1.5 | 13.8 ± 1.6 | 12.4 ± 2.7 |
| % of connective tissue and necrosis area with respect to total tumor area | 77.3 | 18.5 | 11.1 |
| . | Treatment . | ||
|---|---|---|---|
| L19–IL2 . | D1.3–IL2 . | Saline . | |
| Weight of tumors (g) | 0.05 ± 0.01 | 0.597 ± 0.1 | 0.811 ± 0.23 |
| TILs* | 53.2 ± 13.8 | 0.75 ± 0.14 | 0.52 ± 0.26 |
| M/HPF | 5.2 ± 1.5 | 13.8 ± 1.6 | 12.4 ± 2.7 |
| % of connective tissue and necrosis area with respect to total tumor area | 77.3 | 18.5 | 11.1 |
The nonparametric Mann-Whitney test was used to determine the statistical significance of differences in tumor weights among the three groups of animals. The differences in tumor weights following treatment with the fusion protein L19–IL-2 were statistically significant compared to treatment with phosphate-buffered saline (PBS) or D1.3–IL-2 fusion protein (L19–IL-2/D1.3–IL-2, P = .003; L19–IL-2/PBS, P = .003; D1.3–IL-2/PBS:P = .423).
TIL indicates tumor-infiltrating lymphocytes; M/HPF, mitoses per high-power field.
The quantitative evaluation of CD45R/B220+ cells was carried out using a rat antimouse CD45/B220 in immunohistochemistry. Values reported represent the mean number of positive TIL/HPF ± SE.