Antibodies used along with pretreatments and dilutions
| Antibody and clone . | Source . | Pretreatment . | Dilution . |
|---|---|---|---|
| bcl-2 clone 124 | DAKO M887 | M/W | 1:50 |
| Anti-ki-67, clone MIB-1 | Immunotech 0505 | M/W | 1:50 |
| Anti-P53 protein, clone DO7 | HMDS, hybridoma kindly donated by Prof. D. Lane | M/W | Neat |
| NCL-CD10-270 | Novacastra | M/W (TSA) | 1:50 |
| CD23 | Dako | M/W (TSA) | 1:20 |
| CDw75 (NCL-LN1) | Novacastra | M/W × 2 | 1:20 |
| Anti-bcl6 N-3 | Santa Cruz | M/W × 2 | 1:10 |
| Antibody and clone . | Source . | Pretreatment . | Dilution . |
|---|---|---|---|
| bcl-2 clone 124 | DAKO M887 | M/W | 1:50 |
| Anti-ki-67, clone MIB-1 | Immunotech 0505 | M/W | 1:50 |
| Anti-P53 protein, clone DO7 | HMDS, hybridoma kindly donated by Prof. D. Lane | M/W | Neat |
| NCL-CD10-270 | Novacastra | M/W (TSA) | 1:50 |
| CD23 | Dako | M/W (TSA) | 1:20 |
| CDw75 (NCL-LN1) | Novacastra | M/W × 2 | 1:20 |
| Anti-bcl6 N-3 | Santa Cruz | M/W × 2 | 1:10 |
M/W indicates microwave antigen retrieval (400 mL of citrate buffer pH 6.0 on high power (800 W) for 12 minutes); M/W × 2, M/W plus an additional 5 minutes following a 5-minute rest.