Expression of IL-13 and IL-5 in the CD56+ and CD56− natural killer cell subsets
| Culture conditions* . | (CD161/CD56)+cells† . | IFN-γ . | IL-5 . | IL-13 . |
|---|---|---|---|---|
| Flt3-L + IL-2 | CD56− | 2 ± 3‡ | 21 ± 92-153 | 74 ± 212-153 |
| CD56+ | 11 ± 9 | 1 ± 1 | 8 ± 6 |
| Culture conditions* . | (CD161/CD56)+cells† . | IFN-γ . | IL-5 . | IL-13 . |
|---|---|---|---|---|
| Flt3-L + IL-2 | CD56− | 2 ± 3‡ | 21 ± 92-153 | 74 ± 212-153 |
| CD56+ | 11 ± 9 | 1 ± 1 | 8 ± 6 |
IFN-γ indicates interferon gamma.
Flt3-L and IL-2 were added to primary cultures of CD34+ cells, as described in “Materials and methods.”
Expression of the cytokines listed on top was analyzed (4-color immunofluorescence) in cells stimulated as described in “Materials and methods.” Analysis was performed on gated CD3−/(CD161/CD56)+ and CD3−/CD56+ cells. The proportion of CD56+ cells within the CD161+ natural killer cells were 40% ± 25% (mean ± SD, n = 3). The proportion of cells producing cytokines within the CD161+/CD56− cells was calculated as described in “Materials and methods.”
Numbers are percent positive cells (mean ± SD, n = 3).
Percentage positive cells in the CD56−population significantly different (P < .05) from that in the CD56+ population.