Cytokine expression in CD56+ and CD56−, CD161+ natural killer cells generated in primary cultures of Lin− cells
| Culture conditions* . | CD161+cells† . | IFN-γ %‡ . | GM-CSF . | TNF-α . | IL-5 % . | ||
|---|---|---|---|---|---|---|---|
| % . | MFI1-153 . | % . | MFI . | ||||
| rIL-2 | CD56− | < 0.5* (6)1-155 | 31.3 ± 19.5 (4) | 3.7 ± 1.7 (4) | 25.7 ± 6.9 (4) | 5.1 ± 1.2 (4) | 5.5 ± 4.71-154 (4) |
| CD56+ | 6.9 ± 2.7 (6) | 11.6 ± 7.7 (4) | 2.8 ± 1.2 (4) | 16.8 ± 7.9 (4) | 4.7 ± 1.3 (4) | < 0.5 (4) | |
| rIL-15 | CD56+ | 8.7 ± 3.9 (3) | 18.6 ± 13.2 (3) | 1.4 ± 0.3 (3) | 19.0 ± 4.6 (3) | 3.4 ± 0.1 (3) | < 0.5 (3) |
| Culture conditions* . | CD161+cells† . | IFN-γ %‡ . | GM-CSF . | TNF-α . | IL-5 % . | ||
|---|---|---|---|---|---|---|---|
| % . | MFI1-153 . | % . | MFI . | ||||
| rIL-2 | CD56− | < 0.5* (6)1-155 | 31.3 ± 19.5 (4) | 3.7 ± 1.7 (4) | 25.7 ± 6.9 (4) | 5.1 ± 1.2 (4) | 5.5 ± 4.71-154 (4) |
| CD56+ | 6.9 ± 2.7 (6) | 11.6 ± 7.7 (4) | 2.8 ± 1.2 (4) | 16.8 ± 7.9 (4) | 4.7 ± 1.3 (4) | < 0.5 (4) | |
| rIL-15 | CD56+ | 8.7 ± 3.9 (3) | 18.6 ± 13.2 (3) | 1.4 ± 0.3 (3) | 19.0 ± 4.6 (3) | 3.4 ± 0.1 (3) | < 0.5 (3) |
IFN-γ indicates interferon gamma; GM-CSF, granulocyte macrophage–colony-stimulating factor; TNF-α, tumor necrosis factor alpha; MFI, mean fluorescence intensity.
The indicated cytokines were added to primary cultures of Lin− cells with Sl/Sl4hSCF220feeder cells, as described in “Materials and methods.”
Expression of the cytokines listed on top was analyzed (3-color immunofluorescence) in cells stimulated with PMA and Ca2+ ionophore. Analysis was performed on cells gated on the basis of their reactivity with anti-CD161 or anti-CD56 mAb, as described in “Materials and methods,” and Figure 3.
Percent positive cells (mean ± SD).
Fluorescence intensity (mean channel of fluorescence, mean ± SD).
Number of experiments.
Percentage positive cells in the CD56−population significantly different (P < .05) from that in the CD56+ population.