Hematopoietic potential of blast cell colonies grown in the presence of PEG-rhuMGDF alone
| Cells . | Stimuli . | CFU-MK . | BFU-E . | CFU-GM . | CFU-mixed . | Total . |
|---|---|---|---|---|---|---|
| mpl−/−flmpl | VEGF | 144 ± 25 | 32 ± 8 | 36 ± 9 | 11.3 ± 2.7 | 237 ± 20 |
| PEG-rhuMGDF | 127 ± 23 | 49 ± 20 | 37 ± 11 | 18.3 ± 7.3 | 231 ± 50 | |
| mpl−/−Δ3mpl | VEGF | 101 ± 16 | 66 ± 9 | 5.3 ± 2.0 | 20 ± 5.0 | 193 ± 30 |
| PEG-rhuMGDF | 87 ± 15 | 60 ± 14 | 14.3 ± 2.4 | 11.3 ± 2.3 | 172 ± 24 |
| Cells . | Stimuli . | CFU-MK . | BFU-E . | CFU-GM . | CFU-mixed . | Total . |
|---|---|---|---|---|---|---|
| mpl−/−flmpl | VEGF | 144 ± 25 | 32 ± 8 | 36 ± 9 | 11.3 ± 2.7 | 237 ± 20 |
| PEG-rhuMGDF | 127 ± 23 | 49 ± 20 | 37 ± 11 | 18.3 ± 7.3 | 231 ± 50 | |
| mpl−/−Δ3mpl | VEGF | 101 ± 16 | 66 ± 9 | 5.3 ± 2.0 | 20 ± 5.0 | 193 ± 30 |
| PEG-rhuMGDF | 87 ± 15 | 60 ± 14 | 14.3 ± 2.4 | 11.3 ± 2.3 | 172 ± 24 |
Mpl−/− flmpl and mpl−/−Δ3mpl-derived blast cell colonies grown in the presence of PEG-rhuMGDF were analyzed for their content in myeloid progenitors with a combination of 6GF and compared to that of blast cell colonies grown in the presence of VEGF. Results represent the mean number of progenitors per 105 cells of blast cell colonies (duplicate dishes in 3 independent experiments).