Effect of nystatin and filipin on caveolae-dependent internalization
| Cells . | Chemokine . | Control . | Inhibitor . | |
|---|---|---|---|---|
| Filipin . | Nystatin . | |||
| CHO.CCR5 | MIP-1α | 50.8 ± 3.2 (6) | 64.5 ± 4.5 (5)* | 97.2 ± 8.8 (6)† |
| RANTES | 54.4 ± 3.1 (5) | 63.9 ± 4.3 (6) | 84.0 ± 4.2 (6)† | |
| MIP-1β | 85.6 ± 3.7 (6) | 95.0 ± 3.6 (3) | 91.0 ± 4.6 (3) | |
| CHO.CCR5.CD4 | MIP-1α | 48.6 ± 3.6 (7) | 111.2 ± 2.4 (4)† | 70.8 ± 3.7 (5)‡ |
| RANTES | 59.6 ± 4.8 (8) | 77.4 ± 3.2 (8)† | 77.7 ± 4.9 (8)‡ | |
| MIP-1β | 42.2 ± 10.9 (5) | 98.5 ± 6.1 (9)‡ | 92.7 ± 6.9 (9)‡ | |
| Cells . | Chemokine . | Control . | Inhibitor . | |
|---|---|---|---|---|
| Filipin . | Nystatin . | |||
| CHO.CCR5 | MIP-1α | 50.8 ± 3.2 (6) | 64.5 ± 4.5 (5)* | 97.2 ± 8.8 (6)† |
| RANTES | 54.4 ± 3.1 (5) | 63.9 ± 4.3 (6) | 84.0 ± 4.2 (6)† | |
| MIP-1β | 85.6 ± 3.7 (6) | 95.0 ± 3.6 (3) | 91.0 ± 4.6 (3) | |
| CHO.CCR5.CD4 | MIP-1α | 48.6 ± 3.6 (7) | 111.2 ± 2.4 (4)† | 70.8 ± 3.7 (5)‡ |
| RANTES | 59.6 ± 4.8 (8) | 77.4 ± 3.2 (8)† | 77.7 ± 4.9 (8)‡ | |
| MIP-1β | 42.2 ± 10.9 (5) | 98.5 ± 6.1 (9)‡ | 92.7 ± 6.9 (9)‡ | |
Cells were treated with the inhibitors nystatin and filipin, as described, before internalization was induced with MIP-1α, RANTES, or MIP-1β, respectively. Cells that were not treated with inhibitor served as control for internalization. Data represent mean ± SEM of at least 3 independent experiments.
P < .05, relative to control.
P < .001, relative to control.
P < .01, relative to control.