Effect of sucrose on clathrin-coated pit-dependent internalization
| Cells . | Chemokine . | Control . | Sucrose . |
|---|---|---|---|
| CHO.CCR5 | MIP-1α | 50.8 ± 3.2 (6) | 101.4 ± 14.7 (6)* |
| RANTES | 54.4 ± 3.1 (5) | 79.7 ± 17.5 (6) | |
| MIP-1β | 85.6 ± 3.7 (6) | 99.0 ± 5.2 (3) | |
| CHO.CCR5.CD4 | MIP-1α | 48.6 ± 3.6 (7) | 76.9 ± 3.9 (6)† |
| RANTES | 59.6 ± 4.8 (8) | 69.5 ± 3.5 (8)† | |
| MIP-1β | 42.2 ± 10.9 (5) | 94.8 ± 4.5 (9)‡ | |
| HeLaRC49 | MIP-1α | 58.6 ± 4.9 (6) | 97.9 ± 6.1 (6)† |
| Cells . | Chemokine . | Control . | Sucrose . |
|---|---|---|---|
| CHO.CCR5 | MIP-1α | 50.8 ± 3.2 (6) | 101.4 ± 14.7 (6)* |
| RANTES | 54.4 ± 3.1 (5) | 79.7 ± 17.5 (6) | |
| MIP-1β | 85.6 ± 3.7 (6) | 99.0 ± 5.2 (3) | |
| CHO.CCR5.CD4 | MIP-1α | 48.6 ± 3.6 (7) | 76.9 ± 3.9 (6)† |
| RANTES | 59.6 ± 4.8 (8) | 69.5 ± 3.5 (8)† | |
| MIP-1β | 42.2 ± 10.9 (5) | 94.8 ± 4.5 (9)‡ | |
| HeLaRC49 | MIP-1α | 58.6 ± 4.9 (6) | 97.9 ± 6.1 (6)† |
Cells were treated with internalization inhibitor sucrose, as described, before internalization was induced with MIP-1α, RANTES, or MIP-1β, respectively. Cells that were not treated with inhibitor served as control for internalization. Data represent mean ± SEM of at least 3 independent experiments.
P < .01, relative to control.
P < .001, relative to control.
P < .05, relative to control.