Table 3. Neutralization of endogenous... | American Society of Hematology

Table 3.

Neutralization of endogenous SDF-1 affects PB Inc⁻CD34⁺ progenitor survival

Source of cells	Incubation time, h	Total cell number (× 10³) Anti-SDF-1 treatment
Source of cells	Incubation time, h	−	+
Inc⁺
Total CD34⁺	0	100	100
	48	96 ± 2.5	98.5 ± 1.2
	96	68.4 ± 9.2^3-151	65.8 ± 12.7
Inc⁻
Total CD34⁺	0	100	100
	48	95.7 ± 2.8	88.4 ± 3.7
	96	80.4 ± 5.8	50.2 ± 5.1^3-150
Sorted CD34⁺CD38⁺	0	100	100
	72	78.1 ± 7.2	47.6 ± 4.8^3-150
Sorted CD34⁺CD38⁻	0	100	100
	72	48.6 ± 6.6	45.4 ± 5.8

Source of cells	Incubation time, h	Total cell number (× 10³) Anti-SDF-1 treatment
Source of cells	Incubation time, h	−	+
Inc⁺
Total CD34⁺	0	100	100
	48	96 ± 2.5	98.5 ± 1.2
	96	68.4 ± 9.2^3-151	65.8 ± 12.7
Inc⁻
Total CD34⁺	0	100	100
	48	95.7 ± 2.8	88.4 ± 3.7
	96	80.4 ± 5.8	50.2 ± 5.1^3-150
Sorted CD34⁺CD38⁺	0	100	100
	72	78.1 ± 7.2	47.6 ± 4.8^3-150
Sorted CD34⁺CD38⁻	0	100	100
	72	48.6 ± 6.6	45.4 ± 5.8

PB CD34⁺ cells were purified immediately after density gradient separation (Inc⁻) or after incubation on a plastic support (Inc⁺). In some experiments, Inc⁻ cells were sorted according to their CD38 antigen expression. Cells (1 × 10⁵/mL) were cultured under apoptosis-inducing culture conditions (serum- and cytokine-free medium [IMDM]). The role of intracellular SDF-1 in the control of CD34⁺ cell autonomous survival was evaluated by adding an anti–SDF-1 neutralizing antibody (10 ng/mL) to the culture. At various time points, cells were harvested, counted, and processed for viability by trypan blue exclusion. Total cell numbers are presented as the means ± SD of 3 independent experiments.

F3-150

P < .003 versus anti–SDF-1 untreated control.

F3-151

P = .02 versus Inc⁻ cells.

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