Table 2. Production of SDF-1 by CD34... | American Society of Hematology

Table 2.

Production of SDF-1 by CD34⁺ cells is increased under apoptosis-inducing conditions

Source of cells	Apoptosis-inducing incubation time, h	SDF-1 levels, pg/mL
Inc⁺
CD34⁺CD38⁻	2	bsd
	72	bsd
CD34⁺CD38⁺	2	bsd
	72	28.2 ± 12.6^*
Inc⁻
CD34⁺CD38⁻	2	bsd
	72	bsd
CD34⁺CD38⁺	2	25.5 ± 12.6
	72	164.6 ± 19.4^*
CD34⁺[G₀]	2	bsd
	72	bsd
CD34⁺[G₁ + S + G₂/M]	2	40 ± 10.2
	72	206.6 ± 49.8^*

Source of cells	Apoptosis-inducing incubation time, h	SDF-1 levels, pg/mL
Inc⁺
CD34⁺CD38⁻	2	bsd
	72	bsd
CD34⁺CD38⁺	2	bsd
	72	28.2 ± 12.6^*
Inc⁻
CD34⁺CD38⁻	2	bsd
	72	bsd
CD34⁺CD38⁺	2	25.5 ± 12.6
	72	164.6 ± 19.4^*
CD34⁺[G₀]	2	bsd
	72	bsd
CD34⁺[G₁ + S + G₂/M]	2	40 ± 10.2
	72	206.6 ± 49.8^*

PB CD34⁺ cells were purified immediately after density gradient separation (Inc⁻) or after incubation on a plastic support (Inc⁺) and were sorted according to CD38 antigen expression or to their cell cycle status based on Hoechst and pyronin Y staining. Cells (1.5 × 10⁵ cells/mL) were incubated under apoptosis-inducing culture conditions (serum- and cytokine-free medium [IMDM]) for 72 hours before harvesting the conditioned media. Secretion of SDF-1 by the different fractions of sorted cells undergoing apoptosis was evaluated by Quantikine human immunoassay according to the manufacturer's protocol. Analysis of the medium collected after 2 hours of incubation was used as control. Levels of SDF-1 are presented as the means ± SD of 3 independent experiments.

The sensitivity of ELISA for SDF-1 was > 18 pg/mL.

Bsd indicates below sensitivity detection.

P < .002 versus 2 hours incubation.

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