Postthaw cell recovery of peripheral blood stem cells from control and sickle trait subjects after cryopreservation in 10% DMSO or 5% DMSO/6% pentastarch
| Variable . | 10% DMSO . | DMSO/pentastarch . | ||||
|---|---|---|---|---|---|---|
| Control subjects, n = 8 . | SCT subjects, n = 8 . | P . | Control subjects, n = 8 . | SCT subjects, n = 8 . | P . | |
| TNC, % | 75.3 (76.8 ± 8.1) | 76.1 (79.5 ± 13.7) | .646 | 82.9 (81.4 ± 4.8) | 73.2 (77.9 ± 19.7) | .637 |
| CD34+, % | 32.9 (31.5 ± 15.1) | 19.2 (20.3 ± 15.1) | .160 | 92.4 (91.5 ± 23.1) | 86.9 (91.4 ± 16.9) | .991 |
| CD3+, % | 15.4 (19.7 ± 13.0) | 17.8 (22.2 ± 15.4) | .545 | 41.2 (41.8 ± 17.6) | 41.2 (44.3 ± 15.9) | .837 |
| CFU-GM4-150, % | 63.7 (126.2 ± 155.8) | 48.9 (54.8 ± 45.7) | .363 | 76.1 (173.2 ± 203.0) | 57.5 (70.6 ± 40.3) | .303 |
| Variable . | 10% DMSO . | DMSO/pentastarch . | ||||
|---|---|---|---|---|---|---|
| Control subjects, n = 8 . | SCT subjects, n = 8 . | P . | Control subjects, n = 8 . | SCT subjects, n = 8 . | P . | |
| TNC, % | 75.3 (76.8 ± 8.1) | 76.1 (79.5 ± 13.7) | .646 | 82.9 (81.4 ± 4.8) | 73.2 (77.9 ± 19.7) | .637 |
| CD34+, % | 32.9 (31.5 ± 15.1) | 19.2 (20.3 ± 15.1) | .160 | 92.4 (91.5 ± 23.1) | 86.9 (91.4 ± 16.9) | .991 |
| CD3+, % | 15.4 (19.7 ± 13.0) | 17.8 (22.2 ± 15.4) | .545 | 41.2 (41.8 ± 17.6) | 41.2 (44.3 ± 15.9) | .837 |
| CFU-GM4-150, % | 63.7 (126.2 ± 155.8) | 48.9 (54.8 ± 45.7) | .363 | 76.1 (173.2 ± 203.0) | 57.5 (70.6 ± 40.3) | .303 |
Values given as median (mean ± SD).
DMSO indicates dimethyl sulfoxide; SCT, sickle cell trait; TNC, total nucleated cell; and CFU-GM, granulocyte-macrophage colony-forming unit.
Control, n = 6.