Human CFUs and hematopoietic lineages recovered from BM of bnx mice 6 to 12 months after transplantation
| Mouse no. . | Culture conditions . | No. human CFUs . | Percentage human cells of each lineage in bnx BM . | ||||
|---|---|---|---|---|---|---|---|
| CD45 . | CD4 . | CD8 . | CD33 . | CD19 . | |||
| 1 | Cytokines only | 18 | 14.3 | 2.6 | 3.4 | 6.5 | 1.2 |
| 2 | 30 | 12.0 | 1.8 | 4.4 | 2.8 | 2.5 | |
| 3 | 51 | 24.2 | 5.0 | 6.4 | 10.1 | 2.1 | |
| 4 | 22 | 6.2 | 1.2 | 2.0 | 3.2 | 0.9 | |
| 5 | 75 | 7.1 | 1.9 | 2.7 | 2.1 | 1.0 | |
| 6 | 63 | 30.0 | 3.4 | 5.7 | 17.9 | 2.9 | |
| 7 | 33 | 15.7 | 3.1 | 3.5 | 7.8 | 0.3 | |
| 8 | 55 | 22.2 | 4.4 | 5.1 | 8.6 | 0.4 | |
| 9 | 38 | 16.3 | 1.5 | 3.2 | 9.5 | 1.1 | |
| 10 | 40 | 5.5 | 0.9 | 1.4 | 2.4 | 0.6 | |
| 11 | 29 | 20.4 | 2.4 | 4.2 | 10.3 | 1.4 | |
| Avg ± SEM− | 41.3 ± 5.4 | 15.8 ± 2.4 | 2.6 ± 0.4 | 3.5 ± 0.5 | 7.4 ± 1.4 | 1.3 ± 0.3 | |
| 12 | Cytokines + Anti–TGF-β antibody | 46 | 21.6 | 3.6 | 5.1 | 10.4 | 2.9 |
| 13 | 25 | 7.7 | 1.5 | 2.4 | 3.0 | 0.4 | |
| 14 | 32 | 26.3 | 5.8 | 7.2 | 11.9 | 1.3 | |
| 15 | 21 | 11.5 | 2.9 | 3.6 | 3.1 | 1.9 | |
| 16 | 66 | 12.4 | 2.3 | 3.8 | 4.8 | 1.4 | |
| 17 | 39 | 12.9 | 3.4 | 4.4 | 3.2 | 0.9 | |
| 18 | 48 | 33.8 | 2.1 | 5.4 | 20.1 | 3.9 | |
| 19 | ND | 14.4 | 2.2 | 3.2 | 6.3 | 1.6 | |
| 20 | 44 | 18.3 | 4.6 | 4.5 | 6.7 | 2.3 | |
| 21 | 74 | 6.2 | 1.5 | 2.0 | 2.3 | 0.3 | |
| 22 | 26 | 27.9 | 4.1 | 8.5 | 11.6 | 2.0 | |
| 23 | 31 | 10.1 | 2.2 | 3.2 | 2.8 | 0.9 | |
| 24 | 28 | 31.5 | 9.7 | 6.6 | 13.0 | 2.2 | |
| 25 | ND | 17.5 | 3.1 | 4.3 | 8.9 | 1.1 | |
| 26 | 15 | 5.0 | 0.8 | 1.6 | 2.1 | 0.4 | |
| 27 | 40 | 21.8 | 3.6 | 4.7 | 9.4 | 1.4 | |
| 28 | 37 | 42.3 | 6.2 | 12.8 | 16.4 | 3.3 | |
| 29 | 93 | 14.3 | 1.9 | 3.5 | 6.1 | 1.5 | |
| 30 | 81 | 10.5 | 1.1 | 2.5 | 5.0 | 1.0 | |
| 31 | 46 | 14.3 | 2.9 | 3.7 | 5.4 | 0.8 | |
| 32 | 42 | 10.8 | 1.4 | 2.6 | 5.1 | 1.0 | |
| Avg ± SEM− | 43.9 ± 4.8 | 17.7 ± 2.1 | 3.7 ± 0.5 | 4.6 ± 0.6 | 7.5 ± 1.1 | 1.5 ± 0.2 | |
| Mouse no. . | Culture conditions . | No. human CFUs . | Percentage human cells of each lineage in bnx BM . | ||||
|---|---|---|---|---|---|---|---|
| CD45 . | CD4 . | CD8 . | CD33 . | CD19 . | |||
| 1 | Cytokines only | 18 | 14.3 | 2.6 | 3.4 | 6.5 | 1.2 |
| 2 | 30 | 12.0 | 1.8 | 4.4 | 2.8 | 2.5 | |
| 3 | 51 | 24.2 | 5.0 | 6.4 | 10.1 | 2.1 | |
| 4 | 22 | 6.2 | 1.2 | 2.0 | 3.2 | 0.9 | |
| 5 | 75 | 7.1 | 1.9 | 2.7 | 2.1 | 1.0 | |
| 6 | 63 | 30.0 | 3.4 | 5.7 | 17.9 | 2.9 | |
| 7 | 33 | 15.7 | 3.1 | 3.5 | 7.8 | 0.3 | |
| 8 | 55 | 22.2 | 4.4 | 5.1 | 8.6 | 0.4 | |
| 9 | 38 | 16.3 | 1.5 | 3.2 | 9.5 | 1.1 | |
| 10 | 40 | 5.5 | 0.9 | 1.4 | 2.4 | 0.6 | |
| 11 | 29 | 20.4 | 2.4 | 4.2 | 10.3 | 1.4 | |
| Avg ± SEM− | 41.3 ± 5.4 | 15.8 ± 2.4 | 2.6 ± 0.4 | 3.5 ± 0.5 | 7.4 ± 1.4 | 1.3 ± 0.3 | |
| 12 | Cytokines + Anti–TGF-β antibody | 46 | 21.6 | 3.6 | 5.1 | 10.4 | 2.9 |
| 13 | 25 | 7.7 | 1.5 | 2.4 | 3.0 | 0.4 | |
| 14 | 32 | 26.3 | 5.8 | 7.2 | 11.9 | 1.3 | |
| 15 | 21 | 11.5 | 2.9 | 3.6 | 3.1 | 1.9 | |
| 16 | 66 | 12.4 | 2.3 | 3.8 | 4.8 | 1.4 | |
| 17 | 39 | 12.9 | 3.4 | 4.4 | 3.2 | 0.9 | |
| 18 | 48 | 33.8 | 2.1 | 5.4 | 20.1 | 3.9 | |
| 19 | ND | 14.4 | 2.2 | 3.2 | 6.3 | 1.6 | |
| 20 | 44 | 18.3 | 4.6 | 4.5 | 6.7 | 2.3 | |
| 21 | 74 | 6.2 | 1.5 | 2.0 | 2.3 | 0.3 | |
| 22 | 26 | 27.9 | 4.1 | 8.5 | 11.6 | 2.0 | |
| 23 | 31 | 10.1 | 2.2 | 3.2 | 2.8 | 0.9 | |
| 24 | 28 | 31.5 | 9.7 | 6.6 | 13.0 | 2.2 | |
| 25 | ND | 17.5 | 3.1 | 4.3 | 8.9 | 1.1 | |
| 26 | 15 | 5.0 | 0.8 | 1.6 | 2.1 | 0.4 | |
| 27 | 40 | 21.8 | 3.6 | 4.7 | 9.4 | 1.4 | |
| 28 | 37 | 42.3 | 6.2 | 12.8 | 16.4 | 3.3 | |
| 29 | 93 | 14.3 | 1.9 | 3.5 | 6.1 | 1.5 | |
| 30 | 81 | 10.5 | 1.1 | 2.5 | 5.0 | 1.0 | |
| 31 | 46 | 14.3 | 2.9 | 3.7 | 5.4 | 0.8 | |
| 32 | 42 | 10.8 | 1.4 | 2.6 | 5.1 | 1.0 | |
| Avg ± SEM− | 43.9 ± 4.8 | 17.7 ± 2.1 | 3.7 ± 0.5 | 4.6 ± 0.6 | 7.5 ± 1.1 | 1.5 ± 0.2 | |
The BM cells were recovered from bnx mice 6 to 12 months after cotransplantation of human hematopoietic cells and IL-3–producing stromal cells. Human-specific colony-forming assays were plated from each bnx/human marrow sample. Colonies were enumerated on day 21. The CFU values shown were obtained from 3 × 105 bnx/human BM cells plated. FACS analyses were done to determine the percentage of human cells of each lineage in the bnx BM. The results shown were the percentages of human cells of each lineage within 10 000 total, ungated bnx/human BM samples analyzed by FACS.