Erythrocyte age distribution in controls and diabetics
| . | Controls . | Diabetics . | ||
|---|---|---|---|---|
| μM phosphorus (%) . | No. of cells, × 109(%) . | μM phosphorus (%) . | No. of cells, × 109 (%) . | |
| Total erythrocytes | 83.1 ± 3.7 (100) | 99.1 ± 6.9 (100) | 75.5 ± 4.2 (100) | 96.6 ± 4.9 (100) |
| Young erythrocytes | 11.6 ± 0.5 (14.6) | 13.4 ± 0.7 (14.6) | 24.1 ± 1.3 (34.9) | 34.3 ± 2.0 (38.2) |
| Average aged erythrocytes | 52.4 ± 2.8 (65.7) | 59.8 ± 2.7 (64.9) | 41.5 ± 2.6 (60.2) | 49.5 ± 3.5 (55.2) |
| Senescent erythrocytes | 15.6 ± 0.9 (19.7) | 18.9 ± 1.3 (20.5) | 3.41 ± 0.19 (4.9) | 5.9 ± 0.4 (6.6) |
| . | Controls . | Diabetics . | ||
|---|---|---|---|---|
| μM phosphorus (%) . | No. of cells, × 109(%) . | μM phosphorus (%) . | No. of cells, × 109 (%) . | |
| Total erythrocytes | 83.1 ± 3.7 (100) | 99.1 ± 6.9 (100) | 75.5 ± 4.2 (100) | 96.6 ± 4.9 (100) |
| Young erythrocytes | 11.6 ± 0.5 (14.6) | 13.4 ± 0.7 (14.6) | 24.1 ± 1.3 (34.9) | 34.3 ± 2.0 (38.2) |
| Average aged erythrocytes | 52.4 ± 2.8 (65.7) | 59.8 ± 2.7 (64.9) | 41.5 ± 2.6 (60.2) | 49.5 ± 3.5 (55.2) |
| Senescent erythrocytes | 15.6 ± 0.9 (19.7) | 18.9 ± 1.3 (20.5) | 3.41 ± 0.19 (4.9) | 5.9 ± 0.4 (6.6) |
Separation was performed on Percoll discontinuous gradient. Twenty-one milliliters of blood (5.83 × 106 and 6.44 × 106 erythrocytes per microliter for controls and diabetics, respectively), after elimination of contaminant leukocytes and platelets, were resuspended in 25 mL; 4.0 mL (20 × 109-22 × 109 erythrocytes) were loaded on the gradient.
The data are expressed as mean ± SD of 5 experiments.