Release of sialic acid from erythrocyte resealed membranes after neutral sialidase action on endogenous substrates
| . | Membrane-bound sialic acid before treatment . | Membrane-bound sialic acid after treatment . | Sialic acid released . | % of release . |
|---|---|---|---|---|
| Controls | ||||
| Total | 48.9 ± 10.6 | 38.5 ± 6.3 | 10.4 ± 0.8 | 21.2 |
| Protein-bound | 43.0 ± 1.8 | 34.7 ± 2.8 | 8.3 ± 0.8 | 19.3 |
| Lipid-bound | 3.2 ± 0.3 | 2.9 ± 0.1 | 2.0 ± 0.1 | 64.0 |
| Diabetics | ||||
| Total | 81.3 ± 19.3 | 76.2 ± 9.1 | 5.1 ± 0.7 | 6.3 |
| Protein-bound | 71.6 ± 12.5 | 68.1 ± 6.6 | 3.5 ± 0.4 | 4.9 |
| Lipid-bound | 8.0 ± 1.5 | 6.3 ± 1.0 | 1.6 ± 0.1 | 20.4 |
| . | Membrane-bound sialic acid before treatment . | Membrane-bound sialic acid after treatment . | Sialic acid released . | % of release . |
|---|---|---|---|---|
| Controls | ||||
| Total | 48.9 ± 10.6 | 38.5 ± 6.3 | 10.4 ± 0.8 | 21.2 |
| Protein-bound | 43.0 ± 1.8 | 34.7 ± 2.8 | 8.3 ± 0.8 | 19.3 |
| Lipid-bound | 3.2 ± 0.3 | 2.9 ± 0.1 | 2.0 ± 0.1 | 64.0 |
| Diabetics | ||||
| Total | 81.3 ± 19.3 | 76.2 ± 9.1 | 5.1 ± 0.7 | 6.3 |
| Protein-bound | 71.6 ± 12.5 | 68.1 ± 6.6 | 3.5 ± 0.4 | 4.9 |
| Lipid-bound | 8.0 ± 1.5 | 6.3 ± 1.0 | 1.6 ± 0.1 | 20.4 |
Sialic acid is expressed as μg/mg of erythrocyte membrane protein.
The data reported are mean ± SD of 7 experiments.