Sialidase release from erythrocyte resealed membranes of controls and type 2 diabetic patients after exhaustive treatment with specific bacterial phospholipase C
| . | Controls . | Diabetic patients . | ||||
|---|---|---|---|---|---|---|
| Resealed membranes . | Supernatant . | Pellet . | Resealed membranes . | Supernatant . | Pellet . | |
| Total protein, mg | 2.5 | 0.30 ± 0.01 | 2.0 ± 0.1 | 2.5 | 0.50 ± 0.02 | 2.0 ± 0.1 |
| % | 100 | 12 | 80 | 100 | 19.5 | 79.5 |
| Acidic sialidase | ||||||
| Specific activity | 6.3 ± 2.1 | 46.2 ± 3.7 | ND | 11.7 ± 3.9 | 15.2 ± 1.7 | 8.5 ± 0.8 |
| Total activity | 15.8 ± 5.3 | 13.9 ± 1.1 | ND | 29.2 ± 9.3 | 7.6 ± 0.8 | 17.0 ± 1.7 |
| % | 100 | 88 | 100 | 26 | 58.2 | |
| Neutral sialidase | ||||||
| Specific activity | 9.8 ± 3.2 | ND | 12.2 ± 1.8 | 8.17 ± 3.90 | ND | 9.83 ± 2.02 |
| Total activity | 24.5 ± 8.6 | ND | 24.4 ± 3.7 | 20.4 ± 10.0 | ND | 19.7 ± 4.0 |
| % | 100 | 99.6 | 100 | 96.6 | ||
| . | Controls . | Diabetic patients . | ||||
|---|---|---|---|---|---|---|
| Resealed membranes . | Supernatant . | Pellet . | Resealed membranes . | Supernatant . | Pellet . | |
| Total protein, mg | 2.5 | 0.30 ± 0.01 | 2.0 ± 0.1 | 2.5 | 0.50 ± 0.02 | 2.0 ± 0.1 |
| % | 100 | 12 | 80 | 100 | 19.5 | 79.5 |
| Acidic sialidase | ||||||
| Specific activity | 6.3 ± 2.1 | 46.2 ± 3.7 | ND | 11.7 ± 3.9 | 15.2 ± 1.7 | 8.5 ± 0.8 |
| Total activity | 15.8 ± 5.3 | 13.9 ± 1.1 | ND | 29.2 ± 9.3 | 7.6 ± 0.8 | 17.0 ± 1.7 |
| % | 100 | 88 | 100 | 26 | 58.2 | |
| Neutral sialidase | ||||||
| Specific activity | 9.8 ± 3.2 | ND | 12.2 ± 1.8 | 8.17 ± 3.90 | ND | 9.83 ± 2.02 |
| Total activity | 24.5 ± 8.6 | ND | 24.4 ± 3.7 | 20.4 ± 10.0 | ND | 19.7 ± 4.0 |
| % | 100 | 99.6 | 100 | 96.6 | ||
Sialidase specific and total activities are expressed as μU/mg protein and μU/total protein, respectively.
The data are expressed as mean ± SD of 5 experiments.
ND indicates not detectable.