Differential effects of selected chemokines on the cycling of various progenitors in the adherent layer of human LTCs
| Factor added . | Killed by3H-thymidine (%) . | ||
|---|---|---|---|
| Primitive BFU-Es . | CFU-GMs . | ||
| Primitive . | Mature . | ||
| None | 52 ± 4 | 50 ± 4 | 49 ± 1 |
| SDF-1 | 0 ± 3 | 0 ± 2 | 45 ± 3 |
| HCC-1 | 8 ± 3 | 0 ± 2 | 38 ± 2 |
| I-309 | 65 ± 12 | 28 ± 0 | 46 ± 1 |
| Factor added . | Killed by3H-thymidine (%) . | ||
|---|---|---|---|
| Primitive BFU-Es . | CFU-GMs . | ||
| Primitive . | Mature . | ||
| None | 52 ± 4 | 50 ± 4 | 49 ± 1 |
| SDF-1 | 0 ± 3 | 0 ± 2 | 45 ± 3 |
| HCC-1 | 8 ± 3 | 0 ± 2 | 38 ± 2 |
| I-309 | 65 ± 12 | 28 ± 0 | 46 ± 1 |
All chemokines were added simultaneously with a half-medium change to give a final concentration of 100 ng/mL, and their effects assessed 2 to 3 days later. Results shown are the mean ± SEM of data obtained from 2 to 10 independent experiments. A significant effect (P < .0001) on the cycling of the primitive BFU-Es and primitive CFU-GMs was observed when SDF-1 or HCC-1 was added. A slight effect (P < .05) on the cycling of the primitive CFU-GMs (only) was seen when I-309 was added. There was no significant effect of any of the chemokines tested on the cycling of the mature CFU-GMs. The total colony counts in the control groups (no3H-thymidine) ranged from 12 to 385.