Lentiviral transduction of PB-derived CD34+cells and lymphohematopoietic reconstitution of NOD/SCID mice at MOIs of 10 and 50
| . | Mock . | MOI = 10 . | MOI = 50 . |
|---|---|---|---|
| In vitro culture | |||
| CD34+ | 82 | 80 | 81 |
| eGFP+cells | 0 | 42 ± 5 | 36 |
| NOD/SCID repopulation | |||
| No. of mice | 6 | 10 | 3 |
| hu CD45+ | 48.0 ± 20.0 | 29.0 ± 23.9 | 45.3 ± 33.1 |
| hu eGFP+/CD45+ | 0.8 ± 0.2 | 40.8 ± 6.2 | 32.3 ± 2.6 |
| CD34+ | 8.4 ± 2.8 | 12.4 ± 3.4 | 14.3 ± 5.4 |
| eGFP/CD34+ | 38.0 ± 10.4 | 28.3 ± 3.1 | |
| CD33+ | 38.0 ± 20.1 | 43.7 ± 13.6 | 69.0 ± 2.9 |
| eGFP/CD33+ | 48.2 ± 6.0 | 33.0 ± 4.6 | |
| CD38+ | 57.3 ± 9.2 | 68.5 ± 9.6 | 65.3 ± 4.5 |
| eGFP/CD38+ | 45.2 ± 5.5 | 35.0 ± 2.8 | |
| CD19+ | 32.7 ± 1.2 | 36.7 ± 4.7 | 21.3 ± 4.5 |
| eGFP/CD19+ | 44.1 ± 6.0 | 38.7 ± 5.8 |
| . | Mock . | MOI = 10 . | MOI = 50 . |
|---|---|---|---|
| In vitro culture | |||
| CD34+ | 82 | 80 | 81 |
| eGFP+cells | 0 | 42 ± 5 | 36 |
| NOD/SCID repopulation | |||
| No. of mice | 6 | 10 | 3 |
| hu CD45+ | 48.0 ± 20.0 | 29.0 ± 23.9 | 45.3 ± 33.1 |
| hu eGFP+/CD45+ | 0.8 ± 0.2 | 40.8 ± 6.2 | 32.3 ± 2.6 |
| CD34+ | 8.4 ± 2.8 | 12.4 ± 3.4 | 14.3 ± 5.4 |
| eGFP/CD34+ | 38.0 ± 10.4 | 28.3 ± 3.1 | |
| CD33+ | 38.0 ± 20.1 | 43.7 ± 13.6 | 69.0 ± 2.9 |
| eGFP/CD33+ | 48.2 ± 6.0 | 33.0 ± 4.6 | |
| CD38+ | 57.3 ± 9.2 | 68.5 ± 9.6 | 65.3 ± 4.5 |
| eGFP/CD38+ | 45.2 ± 5.5 | 35.0 ± 2.8 | |
| CD19+ | 32.7 ± 1.2 | 36.7 ± 4.7 | 21.3 ± 4.5 |
| eGFP/CD19+ | 44.1 ± 6.0 | 38.7 ± 5.8 |
Data for MOI of 10 are from 2 and for MOI of 50 from a single experiment, all using improved lentiviral vectors.