Lentiviral transduction of PB-derived CD34+cells and lymphohematopoietic reconstitution of NOD/SCID mice at MOI of 3
| . | Mock . | MOI = 3 (+ cytokine) . | MOI = 3 (− cytokine) . |
|---|---|---|---|
| In vitro culture | |||
| CD34+ | 85 | 85 | 84 |
| eGFP+ cells | 0 | 13 | 13 |
| NOD/SCID repopulation | |||
| No. of mice | 3 | 3 | 3 |
| hu CD45+ | 85.0 ± 4.2 | 83.3 ± 4.7 | 78.0 ± 5.7 |
| hu eGFP+/CD45+ | 1.2 ± 0.3 | 12.0 ± 0.4 | 9.2 ± 0.9 |
| CD34+ | 6.0 ± 0.0 | 5.5 ± 0.4 | 5.3 ± 0.8 |
| eGFP/CD34+ | 20.9 ± 1.1 | 18.8 ± 1.2 | |
| CD33+ | 70.5 ± 5.8 | 75.3 ± 2.0 | 74.7 ± 4.5 |
| eGFP/CD33+ | 12.2 ± 0.5 | 10.2 ± 1.2 | |
| CD38+ | 72.5 ± 2.2 | 72.5 ± 0.7 | 66.9 ± 2.9 |
| eGFP/CD38+ | 12.9 ± 1.0 | 9.8 ± 0.3 | |
| CD19+ | 19.0 ± 3.3 | 21.6 ± 0.8 | 20.2 ± 0.5 |
| eGFP/CD19+ | 14.1 ± 1.0 | 11.9 ± 0.3 | |
| CD14+ | 30.6 ± 7.3 | 28.7 ± 0.3 | 27.5 ± 4.2 |
| eGFP/CD14+ | 18.9 ± 1.1 | 16.5 ± 1.5 |
| . | Mock . | MOI = 3 (+ cytokine) . | MOI = 3 (− cytokine) . |
|---|---|---|---|
| In vitro culture | |||
| CD34+ | 85 | 85 | 84 |
| eGFP+ cells | 0 | 13 | 13 |
| NOD/SCID repopulation | |||
| No. of mice | 3 | 3 | 3 |
| hu CD45+ | 85.0 ± 4.2 | 83.3 ± 4.7 | 78.0 ± 5.7 |
| hu eGFP+/CD45+ | 1.2 ± 0.3 | 12.0 ± 0.4 | 9.2 ± 0.9 |
| CD34+ | 6.0 ± 0.0 | 5.5 ± 0.4 | 5.3 ± 0.8 |
| eGFP/CD34+ | 20.9 ± 1.1 | 18.8 ± 1.2 | |
| CD33+ | 70.5 ± 5.8 | 75.3 ± 2.0 | 74.7 ± 4.5 |
| eGFP/CD33+ | 12.2 ± 0.5 | 10.2 ± 1.2 | |
| CD38+ | 72.5 ± 2.2 | 72.5 ± 0.7 | 66.9 ± 2.9 |
| eGFP/CD38+ | 12.9 ± 1.0 | 9.8 ± 0.3 | |
| CD19+ | 19.0 ± 3.3 | 21.6 ± 0.8 | 20.2 ± 0.5 |
| eGFP/CD19+ | 14.1 ± 1.0 | 11.9 ± 0.3 | |
| CD14+ | 30.6 ± 7.3 | 28.7 ± 0.3 | 27.5 ± 4.2 |
| eGFP/CD14+ | 18.9 ± 1.1 | 16.5 ± 1.5 |
The total number of human cells, their subpopulations, and level of eGFP expression are given in percent ± SD. eGFP+/CD34+ represents the percent of eGFP+ cells within the CD34+ human cell fraction.