Agonist and antagonist activity of single amino acid substitutions
| Position . | Substituted amino acids . | 3HA15 . | 5W38 . | ||
|---|---|---|---|---|---|
| Agonist . | Antagonist . | Agonist . | Antagonist . | ||
| 3 (H) | A, Q, L | ++ | − | − | +/− |
| R, K | − | +/− | − | − | |
| N, C, V | ++ | − | +/− | − | |
| D, E, F | ++ | − | − | − | |
| G, S | − | ++ | − | +/− | |
| M, T, I | ++ | − | ++ | − | |
| P, W | − | ++ | − | − | |
| Y | ++ | +/− | − | − | |
| 4 (D) | N, Q, I | − | ++ | − | − |
| H, M, F, W, Y, V | − | +/− | − | − | |
| Rest | − | − | − | − | |
| 5 (D) | All | − | − | − | − |
| 7 (L) | A, F | − | ++ | +/− | − |
| R | − | − | +/− | +/− | |
| N, H | − | +/− | − | − | |
| D | − | − | − | +/− | |
| C | − | ++ | ++ | − | |
| Q | − | +/− | − | +/− | |
| E | +/− | − | +/− | − | |
| G, Y | − | +/− | +/− | − | |
| I, M | +/− | − | ++ | − | |
| K | − | − | ++ | − | |
| P | − | ++ | − | +/− | |
| S, T, V | − | ++ | − | − | |
| W | − | +/− | − | − | |
| 8 (E) | A | +/− | − | − | +/− |
| R, L | − | − | − | − | |
| D | +/− | ++ | ++ | − | |
| C | ++ | − | ++ | − | |
| N, Q, H | − | +/− | − | +/− | |
| G, S | − | ++ | ++ | − | |
| K | ++ | +/− | ++ | − | |
| M | +/− | − | − | − | |
| I, F, P, T, W, Y | − | +/− | − | − | |
| V | − | − | − | +/− | |
| Position . | Substituted amino acids . | 3HA15 . | 5W38 . | ||
|---|---|---|---|---|---|
| Agonist . | Antagonist . | Agonist . | Antagonist . | ||
| 3 (H) | A, Q, L | ++ | − | − | +/− |
| R, K | − | +/− | − | − | |
| N, C, V | ++ | − | +/− | − | |
| D, E, F | ++ | − | − | − | |
| G, S | − | ++ | − | +/− | |
| M, T, I | ++ | − | ++ | − | |
| P, W | − | ++ | − | − | |
| Y | ++ | +/− | − | − | |
| 4 (D) | N, Q, I | − | ++ | − | − |
| H, M, F, W, Y, V | − | +/− | − | − | |
| Rest | − | − | − | − | |
| 5 (D) | All | − | − | − | − |
| 7 (L) | A, F | − | ++ | +/− | − |
| R | − | − | +/− | +/− | |
| N, H | − | +/− | − | − | |
| D | − | − | − | +/− | |
| C | − | ++ | ++ | − | |
| Q | − | +/− | − | +/− | |
| E | +/− | − | +/− | − | |
| G, Y | − | +/− | +/− | − | |
| I, M | +/− | − | ++ | − | |
| K | − | − | ++ | − | |
| P | − | ++ | − | +/− | |
| S, T, V | − | ++ | − | − | |
| W | − | +/− | − | − | |
| 8 (E) | A | +/− | − | − | +/− |
| R, L | − | − | − | − | |
| D | +/− | ++ | ++ | − | |
| C | ++ | − | ++ | − | |
| N, Q, H | − | +/− | − | +/− | |
| G, S | − | ++ | ++ | − | |
| K | ++ | +/− | ++ | − | |
| M | +/− | − | − | − | |
| I, F, P, T, W, Y | − | +/− | − | − | |
| V | − | − | − | +/− | |
Values represent lysis (agonist) and inhibition of lysis (antagonist) activity of single amino acid substitutions at positions 3, 4, 5, 7, and 8 of the HA-1 peptide with CTL clones 3HA15 and 5W38.
− Indicates less than 25% lysis/inhibition of lysis; ±, 25% to 50% lysis/inhibition of lysis; and ++, more than 50% lysis/inhibition of lysis.