Table 2.

List of differentially expressed genes between RhloLin−/loSca-1+c-kit+and RhhiLin−/loSca-1+c-kit+HSCs

NameClone codeAccession numberBackgroundRhloRhhiLibrary
Transcription factor    Fold change Fold change  
 Nfix BA-RA6D21 NM_010906 Nuclear factor I family; role in development 3.5  B 
 Hoxa9 BA-RA1C45 M28449 Homologous to the Drosophila Hoxa9  2.4 B  
 Zfp207 BA-RA1D05 NM_011751 Zinc finger protein Zep; 2 C2H2 ZF, NLS 2.1  B 
 Enx-1 BA-RA1C35 NM_007971 Polycomb family, regulate Hox gene expression  2.5 B 
Kinase       
 PI3 kinase BA-RA5A82 BG354681 Phosphoinositide 3-kinase 2  B 
 PKA-like BA-RA4C80 BG354682 cAMP-dependent protein kinase 3.1  B 
Phosphatase       
 Tyr phosphatase BA-RA1D25 X63440 p19 protein-tyrosine-phosphatase  2.3 B  
RNA binding       
 RBM3 MSC-RA1A25 NM_016809 RNA binding motif 3 4.1  A 
 Napor-3 BA-RA6B66 AF090697 Apoptosis-related RNA-binding protein 2.7  B  
 Poly(a) binding BA-RA6B35 X65553 RNA-binding protein 2.3  B 
Protease       
 Calpain 4 MSC-RA17B72 NM_009795 Calcium-dependent protease 6.2  A  
Cell growth       
 Activin beta C MSC-RA15B58 X82540 TGF-beta family  4.3 A  
 TAX responsive MSC-RA15B86 X81987 Malignancy-related protein 107  3.5 A  
 Inhibitor of CDC42 MSC-RA16A75 L07918 Hematopoietic-specific, GDP dissociation inhibitor  21 A  
Protein modification       
 CCT eta MSC-RA12A61 Z31399 Chaperonin containing TCP-1 4.7  A 
 NEDD-4 MSC-RA16D13 P46935 Containing WW domain 2.1  A  
Membrane associated       
 pRGR1 BA-RA6C77 AF041429 4 TM; unknown function  3.9 B 
 TM-protein BA-RA4C93 AF082835 Type III transmembrane protein  3.7 B  
 Ly-6C.2 MSC-RA11C15 M18466 u-PAR domain proteins 6.1  A  
Nuclear protein       
 PWP1-like BA-RA5C56 L07758 WD-40, nuclear phosphoprotein  2.3 B 
 Npm1 BA-RA4A70 NM_008722 Nucleophosmin 1–related protein  3 B 
 Karyopherin BA-RA1C82 NM_010655 Importin  5.9 B 
Calcium binding       
 PW29 BA-RA4C42 D49429 Calcium-binding protein  3.9 B  
 Cam I BA-RA6C79 M19381 Calmodulin-dependent kinase and phosphatase  5.9 B  
Chromosome modeling       
 HAT1 BA-RA6D08 NM_003642 Histone acetyltransferase 1 2  A/B  
 Histone H2A BA-RA5A12 AF171080 Chromatin structure 3.1  A/B 
 Set beta isoform MSC-RA15B10 S68987 Inhibitor of HAT and PP2a  3.7 A 
 HMG1-related MSC-RA17A09 U00431 Chromatin organization 2  A/B 
Miscellaneous       
 Ant2 BA-RA2D75 AF240003 Adenine nucleotide translocase 2  4.1 B 
 Prg2 MSC-RA14C84 NM_008920 Major basic protein in eosinophils of bone marrow  4.9 A  
 Calponin 2 MSC-RA16D77 NM_007725 Actin binding protein  6.5 A 
 Transport-like proteins BA-RA1D09 AY008297 RPGR-interacting protein 2  A/B 
 CYLN2 BA-RA4A42 AAF99333 Cytoplasmic linker proteins 2  B 
Novel       
 KIAA0780 BA-RA1D26 BG354685 Unknown function  2.4 B  
 RP42 BA-RA2C82 BG354683 Unknown function 2  B  
 PTD011 BA-RA5D01 BG354684 Unknown function 2.9  B 
 Unknown BA-RA3D39 BG354687 Unknown function  2.1 B 
 Unknown MSC-RA16B18 BG354690 Unknown function 5.1  A 
 Unknown BA-RA4A52 BG354679 Unknown function  2.3 B 
 Unknown BA-RA4B58 BG354678 Unknown function 2  B 
 Unknown MSC-RA15C76 BG354689 Unknown function 2  A 
NameClone codeAccession numberBackgroundRhloRhhiLibrary
Transcription factor    Fold change Fold change  
 Nfix BA-RA6D21 NM_010906 Nuclear factor I family; role in development 3.5  B 
 Hoxa9 BA-RA1C45 M28449 Homologous to the Drosophila Hoxa9  2.4 B  
 Zfp207 BA-RA1D05 NM_011751 Zinc finger protein Zep; 2 C2H2 ZF, NLS 2.1  B 
 Enx-1 BA-RA1C35 NM_007971 Polycomb family, regulate Hox gene expression  2.5 B 
Kinase       
 PI3 kinase BA-RA5A82 BG354681 Phosphoinositide 3-kinase 2  B 
 PKA-like BA-RA4C80 BG354682 cAMP-dependent protein kinase 3.1  B 
Phosphatase       
 Tyr phosphatase BA-RA1D25 X63440 p19 protein-tyrosine-phosphatase  2.3 B  
RNA binding       
 RBM3 MSC-RA1A25 NM_016809 RNA binding motif 3 4.1  A 
 Napor-3 BA-RA6B66 AF090697 Apoptosis-related RNA-binding protein 2.7  B  
 Poly(a) binding BA-RA6B35 X65553 RNA-binding protein 2.3  B 
Protease       
 Calpain 4 MSC-RA17B72 NM_009795 Calcium-dependent protease 6.2  A  
Cell growth       
 Activin beta C MSC-RA15B58 X82540 TGF-beta family  4.3 A  
 TAX responsive MSC-RA15B86 X81987 Malignancy-related protein 107  3.5 A  
 Inhibitor of CDC42 MSC-RA16A75 L07918 Hematopoietic-specific, GDP dissociation inhibitor  21 A  
Protein modification       
 CCT eta MSC-RA12A61 Z31399 Chaperonin containing TCP-1 4.7  A 
 NEDD-4 MSC-RA16D13 P46935 Containing WW domain 2.1  A  
Membrane associated       
 pRGR1 BA-RA6C77 AF041429 4 TM; unknown function  3.9 B 
 TM-protein BA-RA4C93 AF082835 Type III transmembrane protein  3.7 B  
 Ly-6C.2 MSC-RA11C15 M18466 u-PAR domain proteins 6.1  A  
Nuclear protein       
 PWP1-like BA-RA5C56 L07758 WD-40, nuclear phosphoprotein  2.3 B 
 Npm1 BA-RA4A70 NM_008722 Nucleophosmin 1–related protein  3 B 
 Karyopherin BA-RA1C82 NM_010655 Importin  5.9 B 
Calcium binding       
 PW29 BA-RA4C42 D49429 Calcium-binding protein  3.9 B  
 Cam I BA-RA6C79 M19381 Calmodulin-dependent kinase and phosphatase  5.9 B  
Chromosome modeling       
 HAT1 BA-RA6D08 NM_003642 Histone acetyltransferase 1 2  A/B  
 Histone H2A BA-RA5A12 AF171080 Chromatin structure 3.1  A/B 
 Set beta isoform MSC-RA15B10 S68987 Inhibitor of HAT and PP2a  3.7 A 
 HMG1-related MSC-RA17A09 U00431 Chromatin organization 2  A/B 
Miscellaneous       
 Ant2 BA-RA2D75 AF240003 Adenine nucleotide translocase 2  4.1 B 
 Prg2 MSC-RA14C84 NM_008920 Major basic protein in eosinophils of bone marrow  4.9 A  
 Calponin 2 MSC-RA16D77 NM_007725 Actin binding protein  6.5 A 
 Transport-like proteins BA-RA1D09 AY008297 RPGR-interacting protein 2  A/B 
 CYLN2 BA-RA4A42 AAF99333 Cytoplasmic linker proteins 2  B 
Novel       
 KIAA0780 BA-RA1D26 BG354685 Unknown function  2.4 B  
 RP42 BA-RA2C82 BG354683 Unknown function 2  B  
 PTD011 BA-RA5D01 BG354684 Unknown function 2.9  B 
 Unknown BA-RA3D39 BG354687 Unknown function  2.1 B 
 Unknown MSC-RA16B18 BG354690 Unknown function 5.1  A 
 Unknown BA-RA4A52 BG354679 Unknown function  2.3 B 
 Unknown BA-RA4B58 BG354678 Unknown function 2  B 
 Unknown MSC-RA15C76 BG354689 Unknown function 2  A 

Libraries A and B were made from Lin−/loSca-1+ and Lin−/loSca-1+Thy-1.1loc-kit+cells, respectively. The changes in expression levels (fold) between Rhlo and Rhhi cells are indicated in the corresponding column of Rhlo and Rhhi. For abbreviations see Table 1.

View Large
Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal