Viable CD34+ cell recoveries after successive cell divisions in the presence or absence of growth factors or STI571
Conditions . | Recovery in M1 (mean %) . | Recovery in M2 (mean %) . | Recovery in M3 (mean %) . | Recovery in M4 (mean %) . | Recovery in M5 (mean %) . | Total recovery (mean %) . |
---|---|---|---|---|---|---|
Total viable cells | ||||||
GFs | 17 | 13 | 26 | 28 | 6 | 91 |
GFs/STI571 | 16 | 15 | 23 | 1.6 | 0.9 | 56 |
No GFs | 14 | 21 | 10 | 1.4 | — | 47 |
No GFs/STI571 | 11 | 9 | 2 | 0.2 | — | 22 |
Total viable CD34+ cells | ||||||
GFs | 11 | 13 | 28 | 28 | 6 | 85 |
GFs/STI571 | 15 | 16 | 23 | 1.6 | 0.9 | 55 |
No GFs | 13 | 25 | 11 | 1.4 | — | 51 |
No GFs/STI571 | 11 | 10 | 2 | 0.2 | — | 24 |
Conditions . | Recovery in M1 (mean %) . | Recovery in M2 (mean %) . | Recovery in M3 (mean %) . | Recovery in M4 (mean %) . | Recovery in M5 (mean %) . | Total recovery (mean %) . |
---|---|---|---|---|---|---|
Total viable cells | ||||||
GFs | 17 | 13 | 26 | 28 | 6 | 91 |
GFs/STI571 | 16 | 15 | 23 | 1.6 | 0.9 | 56 |
No GFs | 14 | 21 | 10 | 1.4 | — | 47 |
No GFs/STI571 | 11 | 9 | 2 | 0.2 | — | 22 |
Total viable CD34+ cells | ||||||
GFs | 11 | 13 | 28 | 28 | 6 | 85 |
GFs/STI571 | 15 | 16 | 23 | 1.6 | 0.9 | 55 |
No GFs | 13 | 25 | 11 | 1.4 | — | 51 |
No GFs/STI571 | 11 | 10 | 2 | 0.2 | — | 24 |
Numbers represent mean (n = 4) percentage recovery for the number of cells in each division peak, corrected for the number of divisions, as a fraction of input cell numbers (see “Materials and methods”).