Cell amplification and recoveries in presence of increasing STI571 concentrations over time
| . | 0 μM STI571 . | 1 μM STI571 . | 5 μM STI571 . | 10 μM STI571 . | |||
|---|---|---|---|---|---|---|---|
| Fold amplification (mean) . | Fold amplification (mean) . | % Recovery (mean) . | Fold amplification (mean) . | % Recovery (mean) . | Fold amplification (mean) . | % Recovery (mean) . | |
| +GFs | |||||||
| Day 3 | 91 ± 36 | 56 ± 20 | 62 | 35 ± 13 | 39 | 50 ± 31 | 55 |
| Day 6 | 538 ± 204 | 192 ± 86 | 36 | 123 ± 62 | 23 | 75 ± 27 | 14 |
| Day 12 | 1167 ± 269 | 1022 ± 372 | 87 | 860 ± 342 | 74 | 478 ± 217 | 41 |
| −GFs | |||||||
| Day 3 | 38 ± 18 | 9 ± 3.1 | 24 | 7 ± 3.3 | 18 | 8 ± 5.1 | 21 |
| Day 6 | 30 ± 21 | 5 ± 1.6 | 17 | 3 ± 1.5 | 10 | 2 ± 1.0 | 7 |
| Day 12 | 162 ± 98 | 10 ± 6.8 | 6 | 5 ± 2.8 | 3 | 4 ± 1.5 | 2.5 |
| . | 0 μM STI571 . | 1 μM STI571 . | 5 μM STI571 . | 10 μM STI571 . | |||
|---|---|---|---|---|---|---|---|
| Fold amplification (mean) . | Fold amplification (mean) . | % Recovery (mean) . | Fold amplification (mean) . | % Recovery (mean) . | Fold amplification (mean) . | % Recovery (mean) . | |
| +GFs | |||||||
| Day 3 | 91 ± 36 | 56 ± 20 | 62 | 35 ± 13 | 39 | 50 ± 31 | 55 |
| Day 6 | 538 ± 204 | 192 ± 86 | 36 | 123 ± 62 | 23 | 75 ± 27 | 14 |
| Day 12 | 1167 ± 269 | 1022 ± 372 | 87 | 860 ± 342 | 74 | 478 ± 217 | 41 |
| −GFs | |||||||
| Day 3 | 38 ± 18 | 9 ± 3.1 | 24 | 7 ± 3.3 | 18 | 8 ± 5.1 | 21 |
| Day 6 | 30 ± 21 | 5 ± 1.6 | 17 | 3 ± 1.5 | 10 | 2 ± 1.0 | 7 |
| Day 12 | 162 ± 98 | 10 ± 6.8 | 6 | 5 ± 2.8 | 3 | 4 ± 1.5 | 2.5 |
Results shown represent mean ± SEM (n = 5) fold amplification based on input 5 × 104 cells per assay.