Results of cloning and sequencing of dominant bands obtained from PCR amplification of TCR-γ chain gene from whole lymph node sections and microdissected CD10+ cells
| Case no. . | PCR band . | Whole lymph node3-150 . | Microdissected CD10+cells3-151 . |
|---|---|---|---|
| 12 | Upper | 4/6 | 6/6 |
| Lower | 3/6 | 6/6 | |
| 26 | Upper | 3/5 | 4/6 |
| Lower | 11/13 | 8/8 | |
| 28 | Upper | 7/9 | 5/7 |
| Lower | 10/10 | Not done |
| Case no. . | PCR band . | Whole lymph node3-150 . | Microdissected CD10+cells3-151 . |
|---|---|---|---|
| 12 | Upper | 4/6 | 6/6 |
| Lower | 3/6 | 6/6 | |
| 26 | Upper | 3/5 | 4/6 |
| Lower | 11/13 | 8/8 | |
| 28 | Upper | 7/9 | 5/7 |
| Lower | 10/10 | Not done |
PCR indicates polymerase chain reaction; TCR-γ, T-cell receptor-γ.
The number of clones with identical TCR-γ chain gene sequence/total number of clones sequenced.
The number of clones with TCR-γ chain gene sequence identical to the dominant sequence obtained from the whole section PCR/total number of clones sequenced.