HTLV-1 tax mRNA load, HTLV-1 proviral DNA load, mRNA/DNA ratio, HTLV-1 Tax-specific CD8+ T-cell frequency, and clinical profiles of HAM/TSP patients and ACs
| Patients . | Age, y . | Sex . | Origin . | tax mRNA load . | Proviral DNA load . | mRNA/DNA ratio . | Tax-specific CD8+ T-cell frequency, % . | EDSS . | Duration of illness, y . |
|---|---|---|---|---|---|---|---|---|---|
| HAM-1 | 38 | F | USA | 0.06 | 1.62 | 3.70 | — | 5.5 | 6 |
| HAM-2 | 47 | F | USA | 1.61 | 11.92 | 13.51 | 0.52 | 6.0 | 10 |
| HAM-3 | 63 | M | USA | 2.32 | 25.16 | 9.22 | 5.70 | 1.5 | 18 |
| HAM-4 | 78 | F | USA | 5.00 | 23.20 | 21.55 | 3.53 | 2.0 | 12 |
| HAM-5 | 46 | F | USA | 5.95 | 12.38 | 48.06 | 4.21 | 3.5 | 12 |
| HAM-6 | 47 | F | Caribbean | 9.76 | 9.37 | 104.16 | — | 6.5 | 2 |
| HAM-7 | 57 | F | USA | 11.79 | 20.98 | 56.20 | — | 6.5 | 3 |
| HAM-8 | 48 | F | USA | 12.32 | 11.70 | 105.30 | — | 6.0 | 4 |
| HAM-9 | 56 | F | USA | 13.62 | 24.76 | 55.01 | — | 6.0 | 13 |
| HAM-10 | 64 | M | USA | 17.78 | 20.67 | 86.02 | — | 6.0 | 12 |
| HAM-11 | 59 | M | Jamaica | 20.08 | 21.99 | 91.31 | 4.71 | 7.0 | 10 |
| HAM-12 | 45 | F | USA | 25.44 | 23.09 | 110.18 | — | 6.0 | 3 |
| HAM-13 | 59 | M | USA | 71.18 | 22.89 | 310.97 | 17.64 | 7.0 | 14 |
| HAM-14 | 44 | M | USA | 109.10 | 47.14 | 231.44 | — | 5.5 | 2 |
| HAM-15 | 79 | M | USA | 137.70 | 69.49 | 198.16 | — | 8.0 | 12 |
| HAM-16 | 49 | M | USA | 187.90 | 60.16 | 312.33 | 24.35 | 8.0 | 6 |
| Mean | 39.48 | 25.41 | 109.82 | 8.67 | |||||
| Median | 12.97* | 22.44† | 88.67‡ | ||||||
| AC-1 | 61 | F | USA | 0.00 | 0.00 | 0.00 | — | — | — |
| AC-2 | 62 | M | USA | 0.00 | 0.07 | 0.00 | — | — | — |
| AC-3 | 49 | F | USA | 0.01 | 0.02 | 50.00 | — | — | — |
| AC-4 | 62 | F | USA | 0.11 | 4.35 | 2.53 | 0.06 | — | — |
| AC-5 | 37 | M | USA | 0.51 | 2.92 | 17.47 | — | — | — |
| AC-6 | 66 | F | USA | 2.87 | 7.97 | 36.01 | 0.45 | — | — |
| AC-7 | 23 | M | USA | 2.94 | 3.61 | 81.44 | 1.64 | — | — |
| AC-8 | 44 | F | Japan | 5.58 | 3.10 | 180.00 | — | — | — |
| Mean | 1.50 | 2.76 | 45.93 | 0.72 | |||||
| Median | 0.31* | 3.01† | 26.74‡ |
| Patients . | Age, y . | Sex . | Origin . | tax mRNA load . | Proviral DNA load . | mRNA/DNA ratio . | Tax-specific CD8+ T-cell frequency, % . | EDSS . | Duration of illness, y . |
|---|---|---|---|---|---|---|---|---|---|
| HAM-1 | 38 | F | USA | 0.06 | 1.62 | 3.70 | — | 5.5 | 6 |
| HAM-2 | 47 | F | USA | 1.61 | 11.92 | 13.51 | 0.52 | 6.0 | 10 |
| HAM-3 | 63 | M | USA | 2.32 | 25.16 | 9.22 | 5.70 | 1.5 | 18 |
| HAM-4 | 78 | F | USA | 5.00 | 23.20 | 21.55 | 3.53 | 2.0 | 12 |
| HAM-5 | 46 | F | USA | 5.95 | 12.38 | 48.06 | 4.21 | 3.5 | 12 |
| HAM-6 | 47 | F | Caribbean | 9.76 | 9.37 | 104.16 | — | 6.5 | 2 |
| HAM-7 | 57 | F | USA | 11.79 | 20.98 | 56.20 | — | 6.5 | 3 |
| HAM-8 | 48 | F | USA | 12.32 | 11.70 | 105.30 | — | 6.0 | 4 |
| HAM-9 | 56 | F | USA | 13.62 | 24.76 | 55.01 | — | 6.0 | 13 |
| HAM-10 | 64 | M | USA | 17.78 | 20.67 | 86.02 | — | 6.0 | 12 |
| HAM-11 | 59 | M | Jamaica | 20.08 | 21.99 | 91.31 | 4.71 | 7.0 | 10 |
| HAM-12 | 45 | F | USA | 25.44 | 23.09 | 110.18 | — | 6.0 | 3 |
| HAM-13 | 59 | M | USA | 71.18 | 22.89 | 310.97 | 17.64 | 7.0 | 14 |
| HAM-14 | 44 | M | USA | 109.10 | 47.14 | 231.44 | — | 5.5 | 2 |
| HAM-15 | 79 | M | USA | 137.70 | 69.49 | 198.16 | — | 8.0 | 12 |
| HAM-16 | 49 | M | USA | 187.90 | 60.16 | 312.33 | 24.35 | 8.0 | 6 |
| Mean | 39.48 | 25.41 | 109.82 | 8.67 | |||||
| Median | 12.97* | 22.44† | 88.67‡ | ||||||
| AC-1 | 61 | F | USA | 0.00 | 0.00 | 0.00 | — | — | — |
| AC-2 | 62 | M | USA | 0.00 | 0.07 | 0.00 | — | — | — |
| AC-3 | 49 | F | USA | 0.01 | 0.02 | 50.00 | — | — | — |
| AC-4 | 62 | F | USA | 0.11 | 4.35 | 2.53 | 0.06 | — | — |
| AC-5 | 37 | M | USA | 0.51 | 2.92 | 17.47 | — | — | — |
| AC-6 | 66 | F | USA | 2.87 | 7.97 | 36.01 | 0.45 | — | — |
| AC-7 | 23 | M | USA | 2.94 | 3.61 | 81.44 | 1.64 | — | — |
| AC-8 | 44 | F | Japan | 5.58 | 3.10 | 180.00 | — | — | — |
| Mean | 1.50 | 2.76 | 45.93 | 0.72 | |||||
| Median | 0.31* | 3.01† | 26.74‡ |
The mRNA/DNA ratio was calculated by the following formula: mRNA/DNA ratio = (HTLV-1 tax mRNA load/HTLV-1 proviral DNA load) × 100.
The Tax-specific CD8+ T-cell frequency is expressed as a percentages of HTLV-1 Tax11-19/HLA-A*0201 tetramer-specific CD8+ T cells in the total amount of CD8+ T cells.
— indicates subjects who are not HLA-A*0201.
HTLV-1 tax mRNA load, HTLV-1 proviral DNA load, and mRNA/DNA ratio were higher in HAM/TSP patients than in ACs with statistical significance by the Mann-Whitney U test:
P = .0014;
P = .0003;
P = .0433. The EDSS score significantly correlated with both HTLV-1 tax mRNA load and mRNA/DNA ratio (P = .0109 and .0089, respectively), but not with HTLV-1 proviral DNA load (P = .5236) by Spearman rank correlation analysis.