Absolute numbers of untransduced and transduced myeloid CFCs* transplanted per mouse
| Mouse group (n) . | Number of myeloid CFCs injected per mouse (×102)† . | |
|---|---|---|
| Untransduced . | G418r or EGFP+ . | |
| Experiment no. 1 | ||
| Neo (4) | 80 | 50 |
| Hoxa9 (4) | 65 | 45 |
| Experiment no. 2 | ||
| EGFP (4) | 180 | 360 |
| Hoxa9-EGFP (4) | 200 | 470 |
| Mouse group (n) . | Number of myeloid CFCs injected per mouse (×102)† . | |
|---|---|---|
| Untransduced . | G418r or EGFP+ . | |
| Experiment no. 1 | ||
| Neo (4) | 80 | 50 |
| Hoxa9 (4) | 65 | 45 |
| Experiment no. 2 | ||
| EGFP (4) | 180 | 360 |
| Hoxa9-EGFP (4) | 200 | 470 |
The number of transduced HSCs injected per mouse can be estimated based on our previous results, which determined the frequency of long-term repopulating cells at 1 per 100 CFCs,18 and estimation of gene transfer to long-term repopulating cells equal to that of CFCs.22
The number of transduced CFCs injected per mouse was determined as follows: (number of bone marrow cells injected per mouse) × (CFC frequency in the injected bone marrow inoculum) × (% of CFCs resistant to G418 or EGFP+).