Colony formation from fetal liver cells by EPO and hGM-CSF
| EPOR . | hGMR . | Cytokine . | No. of colonies/104 fetal liver cells . | ||||
|---|---|---|---|---|---|---|---|
| E . | B . | E-mix . | Non-erythroid . | Total . | |||
| Serum-containing culture | |||||||
| +/+ | −/− | EPO | 488 ± 45 | 0 | 0 | 0 | 488 ± 45 |
| hGM-CSF | 0 | 0 | 0 | 0 | 0 | ||
| +/+ | +/− | EPO | 373 ± 72 | 0 | 0 | 9 ± 6 | 383 ± 78 |
| hGM-CSF | 291 ± 26 | 4 ± 0 | 17 ± 6 | 209 ± 2 | 521 ± 20 | ||
| −/− | −/− | EPO | 0 | 0 | 0 | 0 | 0 |
| hGM-CSF | 0 | 0 | 0 | 4 ± 0 | 4 ± 0 | ||
| −/− | +/− | EPO | 0 | 0 | 0 | 5 ± 2 | 5 ± 2 |
| hGM-CSF | 264 ± 38 | 5 ± 2 | 17 ± 2 | 204 ± 17 | 491 ± 47 | ||
| Serum-free culture | |||||||
| +/+ | +/− | EPO | 189 ± 22 | 0 | 0 | 0 | 189 ± 22 |
| hGM-CSF | 169 ± 19 | 4 ± 0 | 9 ± 6 | 130 ± 23 | 311 ± 27 | ||
| −/− | +/− | EPO | 0 | 0 | 0 | 0 | 0 |
| hGM-CSF | 152 ± 9 | 6 ± 2 | 9 ± 1 | 135 ± 11 | 302 ± 10 | ||
| EPOR . | hGMR . | Cytokine . | No. of colonies/104 fetal liver cells . | ||||
|---|---|---|---|---|---|---|---|
| E . | B . | E-mix . | Non-erythroid . | Total . | |||
| Serum-containing culture | |||||||
| +/+ | −/− | EPO | 488 ± 45 | 0 | 0 | 0 | 488 ± 45 |
| hGM-CSF | 0 | 0 | 0 | 0 | 0 | ||
| +/+ | +/− | EPO | 373 ± 72 | 0 | 0 | 9 ± 6 | 383 ± 78 |
| hGM-CSF | 291 ± 26 | 4 ± 0 | 17 ± 6 | 209 ± 2 | 521 ± 20 | ||
| −/− | −/− | EPO | 0 | 0 | 0 | 0 | 0 |
| hGM-CSF | 0 | 0 | 0 | 4 ± 0 | 4 ± 0 | ||
| −/− | +/− | EPO | 0 | 0 | 0 | 5 ± 2 | 5 ± 2 |
| hGM-CSF | 264 ± 38 | 5 ± 2 | 17 ± 2 | 204 ± 17 | 491 ± 47 | ||
| Serum-free culture | |||||||
| +/+ | +/− | EPO | 189 ± 22 | 0 | 0 | 0 | 189 ± 22 |
| hGM-CSF | 169 ± 19 | 4 ± 0 | 9 ± 6 | 130 ± 23 | 311 ± 27 | ||
| −/− | +/− | EPO | 0 | 0 | 0 | 0 | 0 |
| hGM-CSF | 152 ± 9 | 6 ± 2 | 9 ± 1 | 135 ± 11 | 302 ± 10 | ||
Values are mean ± SD of triplicate plates containing 1 × 104 fetal liver cells of EPO+/+hGMR−/−, EPO+/+hGMR+/−, EPO−/−hGMR−/−, EPO−/−hGMR+/− embryos. The following quantities were used: hGM-CSF, 10 ng/mL; EPO, 2 U/mL.
E indicates erythroid colonies; B, erythroid bursts; E-mix, mixed hematopoietic colonies. See Table 1 for other abbreviations.