Effects of PK stimulators and inhibitors on FANCA phosphorylation
| Agent . | Major target . | Assay . | Effect . |
|---|---|---|---|
| PK stimulator | |||
| cAMP (4 μM) | cAMP-dependent PK | In vitro | − |
| cGMP (4 μM) | cGMP-dependent PK | In vitro | − |
| Calcium (100 μM) | Calmodulin-dependent PK | In vitro | − |
| PMA (100 nM) | Protein kinase C | In vivo† | − |
| PK inhibitor | |||
| cAMP inhibitor protein* (10 U/assay) | cAMP-dependent PK | In vitro | − |
| KT5823 (1 μM) | cGMP-dependent PK | In vitro | − |
| AIP (1 μM) | Calmodulin-dependent PK | In vitro | − |
| Calfostin (500 nM) | Protein kinase C | In vitro | − |
| Hypericin (50 nM) | MAPK, casein kinase II | In vitro | − |
| PD98059 (50 μM) | MAPK kinase | In vivo | − |
| SB203580 (10 μM) | p38 MAPK | In vivo | − |
| Rapamycin (20 nM) | FRAP | In vivo | − |
| Aspirin (5 mM) | IKK-2 | In vivo | − |
| (1 mM) | In vitro | − |
| Agent . | Major target . | Assay . | Effect . |
|---|---|---|---|
| PK stimulator | |||
| cAMP (4 μM) | cAMP-dependent PK | In vitro | − |
| cGMP (4 μM) | cGMP-dependent PK | In vitro | − |
| Calcium (100 μM) | Calmodulin-dependent PK | In vitro | − |
| PMA (100 nM) | Protein kinase C | In vivo† | − |
| PK inhibitor | |||
| cAMP inhibitor protein* (10 U/assay) | cAMP-dependent PK | In vitro | − |
| KT5823 (1 μM) | cGMP-dependent PK | In vitro | − |
| AIP (1 μM) | Calmodulin-dependent PK | In vitro | − |
| Calfostin (500 nM) | Protein kinase C | In vitro | − |
| Hypericin (50 nM) | MAPK, casein kinase II | In vitro | − |
| PD98059 (50 μM) | MAPK kinase | In vivo | − |
| SB203580 (10 μM) | p38 MAPK | In vivo | − |
| Rapamycin (20 nM) | FRAP | In vivo | − |
| Aspirin (5 mM) | IKK-2 | In vivo | − |
| (1 mM) | In vitro | − |