Titration of recombinant retroviral vectors carrying CD2 LCR sequences by G418 selection
| Recombinant retroviral construct . | G418-selection (cfu/mL) . |
|---|---|
| LESN | 6.17 × 104 |
| LESN-LCR | 4.60 × 102 |
| LESNΔ | 7.62 × 104 |
| LESNΔ-LCR | 1.18 × 102 |
| LESNΔ-NLCR | 1.02 × 102 |
| LESNΔ-LCR1 | 4.17 × 104 |
| Recombinant retroviral construct . | G418-selection (cfu/mL) . |
|---|---|
| LESN | 6.17 × 104 |
| LESN-LCR | 4.60 × 102 |
| LESNΔ | 7.62 × 104 |
| LESNΔ-LCR | 1.18 × 102 |
| LESNΔ-NLCR | 1.02 × 102 |
| LESNΔ-LCR1 | 4.17 × 104 |
Based on NIH-3T3 cells. Retroviral vectors were produced in 293T cells by cotransfection of gag-polgpt, HCMV-G, and a genomic RNA-encoding vector. The number of neoR cfu/mL was determined after 2-week selection of NIH-3T3 cells in G418-containing medium (500 μg/mL). Average values reported refer to n = 6-8 independent titration experiments.