Primers used in germline studies
| Exon . | Mutation . | Primers . |
|---|---|---|
| Forward: 5′-TTGTGCCTTTGACCAGAATGTGC-3′ | ||
| Exon 4 | 67 CT | Reverse: 5′-GGGTTACTAATCACACATTTCAAGG-3′ |
| Forward nested: 5′-TGACCAGAATGTGCCTCTAATTG-3′ | ||
| Forward: 5′-CAAATACAAGCTGAAAACTTTGGC-3′ | ||
| Exon 5 | Δ73-76 | Reverse: 5′-ACCTAAAAACAGCATCCCAATTC-3′ |
| Forward nested: 5′-TGACCAGAATGTGCCTCTAATTG-3′ | ||
| Exon 12 | Δ1564- | Forward: 5′-CAAATACAAGCTGAAAACTTTGGC-3′ |
| 1565 | Reverse: 5′-CTCGGCCAAACAAGAAACGCATC-3′ | |
| Forward nested: 5′-GCTGAAAACTTTGGCTTACTTGGA-3′ | ||
| Forward: 5′-CCACACCCGGCCTAAAGTTGTAG-3′ | ||
| Exon 57 | 8150 AT | Reverse: 5′-AAGCAACCTCACTGTACATGTCTG-3′ |
| Forward nested: 5′-GGTGGACCACACAGGAGAATATG-3′ |
| Exon . | Mutation . | Primers . |
|---|---|---|
| Forward: 5′-TTGTGCCTTTGACCAGAATGTGC-3′ | ||
| Exon 4 | 67 CT | Reverse: 5′-GGGTTACTAATCACACATTTCAAGG-3′ |
| Forward nested: 5′-TGACCAGAATGTGCCTCTAATTG-3′ | ||
| Forward: 5′-CAAATACAAGCTGAAAACTTTGGC-3′ | ||
| Exon 5 | Δ73-76 | Reverse: 5′-ACCTAAAAACAGCATCCCAATTC-3′ |
| Forward nested: 5′-TGACCAGAATGTGCCTCTAATTG-3′ | ||
| Exon 12 | Δ1564- | Forward: 5′-CAAATACAAGCTGAAAACTTTGGC-3′ |
| 1565 | Reverse: 5′-CTCGGCCAAACAAGAAACGCATC-3′ | |
| Forward nested: 5′-GCTGAAAACTTTGGCTTACTTGGA-3′ | ||
| Forward: 5′-CCACACCCGGCCTAAAGTTGTAG-3′ | ||
| Exon 57 | 8150 AT | Reverse: 5′-AAGCAACCTCACTGTACATGTCTG-3′ |
| Forward nested: 5′-GGTGGACCACACAGGAGAATATG-3′ |