Definitions of mononuclear cell subsets
| MNC subset . | Definition . |
|---|---|
| B cells | MNCs* expressing CD19 or CD20 and not brightly expressing CD3, CD13, CD14, CD16, CD56, CD10, or CD34 |
| Naive (IgD+) | B cells (defined as above) expressing surface IgD |
| Memory (IgD−) | B cells (defined as above) not expressing surface IgD |
| CD4 T cells (total) | CD3+ CD4+ CD8− MNCs |
| Naive (CD45RAhigh) | MNCs expressing CD4, brightly expressing CD45RA, and not expressing CD8, CD13, CD14, or CD16 |
| Memory or effector (CD45RAlow/−) | MNCs expressing CD4, dimly expressing or not expressing CD45RA, and not expressing CD8, CD13, CD14, or CD16 |
| Costimulation competent (CD28+) | MNCs expressing CD4, expressing CD28, and not expressing CD8, CD13, CD14, or CD16 |
| Costimulation incompetent (CD28−) | MNCs expressing CD4, not expressing CD28, and not expressing CD8, CD13, CD14, or CD16 |
| CD8 T cells (total) | CD3+ CD4− CD8+ MNCs |
| Naive (CD11alow) | MNCs brightly expressing CD8, dimly expressing CD11a, and not expressing CD4, CD13, CD14, CD16, or CD19 |
| Memory or effector (CD11ahigh) | MNCs brightly expressing CD8, brightly expressing CD11a, and not expressing CD4, CD13, CD14, CD16, or CD19 |
| Costimulation competent (CD28+) | MNCs brightly expressing CD8, expressing CD28, and not expressing CD4, CD13, CD14, or CD16 |
| Costimulation incompetent (CD28−) | MNCs brightly expressing CD8, not expressing CD28, and not expressing CD4, CD13, CD14, or CD16 |
| NK cells | MNCs expressing CD16 or CD56 and not expressing CD3 or CD14 |
| Monocytes | CD14+ MNCs |
| MNC subset . | Definition . |
|---|---|
| B cells | MNCs* expressing CD19 or CD20 and not brightly expressing CD3, CD13, CD14, CD16, CD56, CD10, or CD34 |
| Naive (IgD+) | B cells (defined as above) expressing surface IgD |
| Memory (IgD−) | B cells (defined as above) not expressing surface IgD |
| CD4 T cells (total) | CD3+ CD4+ CD8− MNCs |
| Naive (CD45RAhigh) | MNCs expressing CD4, brightly expressing CD45RA, and not expressing CD8, CD13, CD14, or CD16 |
| Memory or effector (CD45RAlow/−) | MNCs expressing CD4, dimly expressing or not expressing CD45RA, and not expressing CD8, CD13, CD14, or CD16 |
| Costimulation competent (CD28+) | MNCs expressing CD4, expressing CD28, and not expressing CD8, CD13, CD14, or CD16 |
| Costimulation incompetent (CD28−) | MNCs expressing CD4, not expressing CD28, and not expressing CD8, CD13, CD14, or CD16 |
| CD8 T cells (total) | CD3+ CD4− CD8+ MNCs |
| Naive (CD11alow) | MNCs brightly expressing CD8, dimly expressing CD11a, and not expressing CD4, CD13, CD14, CD16, or CD19 |
| Memory or effector (CD11ahigh) | MNCs brightly expressing CD8, brightly expressing CD11a, and not expressing CD4, CD13, CD14, CD16, or CD19 |
| Costimulation competent (CD28+) | MNCs brightly expressing CD8, expressing CD28, and not expressing CD4, CD13, CD14, or CD16 |
| Costimulation incompetent (CD28−) | MNCs brightly expressing CD8, not expressing CD28, and not expressing CD4, CD13, CD14, or CD16 |
| NK cells | MNCs expressing CD16 or CD56 and not expressing CD3 or CD14 |
| Monocytes | CD14+ MNCs |
MNC indicates mononuclear cell.
Cells with forward versus side scatter characteristics of lymphocytes and monocytes.