Con A-stimulated CD8+ T lymphocytes from duodenal biopsy specimens of SIVmac-infected rhesus monkeys mediated SIVmac Gag-specific CTL activity
| Monkey3-150 . | Lymphocytes . | Tetramer plus CD8+T cells before culture, %3-151,3-152 . | Tetramer plus CD8+ T cells after culture, %3-151,3-152 . | Lysis3-153 (%) E/T ratio (40/20/10/5/2.5) . |
|---|---|---|---|---|
| 93 | PBL | 1.6 | 5.6 | 58/53/43/26/14 |
| Duodenum3-155 | 1.3 | 1.2 | 14/10/6/4/NT | |
| 94 | PBL | 2.4 | 4.1 | 49/45/32/19/NT |
| Duodenum | 7.3 | 3.2 | NT/20/9/5/5 | |
| 253 | PBL | 4.2 | 2.2 | 26/17/10/6/2 |
| Duodenum | 10.9 | 3.9 | 43/37/25/14/5 | |
| 191 | PBL | 5.3 | 7.1 | NT/NT/NT/NT/NT |
| Duodenum | 7.9 | 4.2 | NT/NT/NT/NT/NT |
| Monkey3-150 . | Lymphocytes . | Tetramer plus CD8+T cells before culture, %3-151,3-152 . | Tetramer plus CD8+ T cells after culture, %3-151,3-152 . | Lysis3-153 (%) E/T ratio (40/20/10/5/2.5) . |
|---|---|---|---|---|
| 93 | PBL | 1.6 | 5.6 | 58/53/43/26/14 |
| Duodenum3-155 | 1.3 | 1.2 | 14/10/6/4/NT | |
| 94 | PBL | 2.4 | 4.1 | 49/45/32/19/NT |
| Duodenum | 7.3 | 3.2 | NT/20/9/5/5 | |
| 253 | PBL | 4.2 | 2.2 | 26/17/10/6/2 |
| Duodenum | 10.9 | 3.9 | 43/37/25/14/5 | |
| 191 | PBL | 5.3 | 7.1 | NT/NT/NT/NT/NT |
| Duodenum | 7.9 | 4.2 | NT/NT/NT/NT/NT |
NT indicates not tested.
Chronically SIVmac251-infected,Mamu-A∗01+ rhesus monkeys.
Cells were cultivated for 10 days with 5 μg/mL Con A and 20 U/mL IL-2.
Staining with PE-coupled tetrameric Mamu-A∗01/p11C, C-M complex on gated CD8αβ+ T lymphocytes.
Percent p11C, C-M-specific lysis was calculated as percent release by p11C, C-M-pulsed, MHC class I-matched target cells minus the average of the percent release by control peptide p11B-pulsed, MHC class I-matched target cells. The background level of p11B peptide-specific lysis was always less than 5% of the spontaneous release.
Lymphocytes from duodenal endoscopic pinch biopsy specimens were isolated by mechanical disruption and enzymatic digestion.