Tetramer-binding CD8+ T lymphocytes in peripheral blood and GI tissues from SIVmac-infected rhesus monkeys
| Monkey* . | PBL . | Tetramer+(%) CD8+ T cells† . | |||
|---|---|---|---|---|---|
| Ileum‡ . | Jejunum‡ . | ||||
| IEL2-153 . | LPL2-155 . | IEL . | LPL . | ||
| 575 | 5.3 | 2.1 | 3.7 | 2.9 | 4.5 |
| 3KI | 1.3 | 0.9 | 3.6 | 0.7 | 1.9 |
| 297 | 6.2 | 3.3 | 10.1 | 3.7 | 14.4 |
| IPI | 6.0 | 0.6 | 3.1 | 0.9 | 4.5 |
| Median | 5.72-154 | 1.52-159 | 3.72-154,2-159 | 1.92-159 | 4.52-154,2-159 |
| Monkey* . | PBL . | Tetramer+(%) CD8+ T cells† . | |||
|---|---|---|---|---|---|
| Ileum‡ . | Jejunum‡ . | ||||
| IEL2-153 . | LPL2-155 . | IEL . | LPL . | ||
| 575 | 5.3 | 2.1 | 3.7 | 2.9 | 4.5 |
| 3KI | 1.3 | 0.9 | 3.6 | 0.7 | 1.9 |
| 297 | 6.2 | 3.3 | 10.1 | 3.7 | 14.4 |
| IPI | 6.0 | 0.6 | 3.1 | 0.9 | 4.5 |
| Median | 5.72-154 | 1.52-159 | 3.72-154,2-159 | 1.92-159 | 4.52-154,2-159 |
Chronically SIVmac 251-infected,Mamu-A∗01+ rhesus monkeys.
Staining with PE-coupled tetrameric Mamu-A∗01/p11C, C-M complex on gated CD8αβ+ T lymphocytes.
Ileal and jejunal tissues were taken from euthanized rhesus monkeys.
IELs were isolated as previously described.10
LPLs were isolated as previously described.10
Median percent tetramer-binding CD8+ T lymphocytes in peripheral blood and LPLs from ileum and jejunum were not significantly different (P = .74; Wilcoxon matched pairs test).
Median percent tetramer-binding CD8+ T lymphocytes in IELs and LPLs from ileum and jejunum were significantly different (P = .0078; Wilcoxon matched pairs test).