Tetramer-binding CD8+ T lymphocytes in peripheral blood and duodenal endoscopic biopsy specimens from SIVmac-infected rhesus monkeys
| Monkey† . | Tetramer+ (%) CD8+ T cells* . | |
|---|---|---|
| PBLs . | Duodenum‡ . | |
| 403 | 9.4 | 4.4 |
| 138 | 4.9 | 3.8 |
| 154 | 13.0 | 9.9 |
| 380 | 3.7 | 9.1 |
| 93 | 1.6 | 1.3 |
| 94 | 2.4 | 7.3 |
| 87 | 1.0 | 9.0 |
| 191 | 5.3 | 7.9 |
| 253 | 4.2 | 10.9 |
| Median1-153 | 4.2 | 7.9 |
| Monkey† . | Tetramer+ (%) CD8+ T cells* . | |
|---|---|---|
| PBLs . | Duodenum‡ . | |
| 403 | 9.4 | 4.4 |
| 138 | 4.9 | 3.8 |
| 154 | 13.0 | 9.9 |
| 380 | 3.7 | 9.1 |
| 93 | 1.6 | 1.3 |
| 94 | 2.4 | 7.3 |
| 87 | 1.0 | 9.0 |
| 191 | 5.3 | 7.9 |
| 253 | 4.2 | 10.9 |
| Median1-153 | 4.2 | 7.9 |
Staining with PE-coupled tetrameric Mamu-A∗01/p11C, C-M complex on gated CD8αβ+ T lymphocytes.
Chronically SIVmac 251-infected,Mamu-A∗01+ rhesus monkeys.
Lymphocytes were isolated from duodenal endoscopic pinch biopsy specimens by mechanical disruption and enzymatic digestion.
Median percent tetramer+ CD8+ T cells in peripheral blood lymphocytes (PBLs) and duodenum were not significantly different (P = .3; Wilcoxon matched pairs test).