The age, diagnosis, percentage of bone marrow blasts, reported karyotype, and allele ratios (lost-retained allele) at tetranucleotide markers D9S1679, D9S746, and D9S761 for the 10 cases of ALL showing loss of heterozygosity
| Case no. . | Age at diagnosis (y, mo) . | ALL type . | % BM blasts . | D9S1679 . | D9S746 . | D9S761 . | Karyotype . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Ratio . | POL . | Ratio . | POL . | Ratio . | POL . | |||||
| 22 | 2, 4 | Pre-B | >99 | 0.17 | M | 0.06 | M | 0.02 | NI | 56-57,XXY,[cp3]/46,XY[9] |
| 20 | 3, 7 | Pre-B | 95 | 0.27 | M | 0.05 | M | 0.02 | NI | 46,XX[2] |
| 29 | 3, 1 | Common | >90 | 0.13 | M | 0.84 | 0.81 | 46,XY[20] | ||
| 12 | 4, 7 | Common | 95 | 0.02 | ni | 0.08 | M | 0 | M | 53-59,XXXX,+6,+21,inc[cp2]/46,XX[8] |
| 16 | 4, 7 | Common | 95 | NI | 0.1 | M | 0.13 | M | 45,XX,der(9)t(9;21)(p11;q11.1)t(9;22)(q34;q11),-21[12]/ 46,XX[3] | |
| 21 | 1, 7 | Common | 81-96 | 0.1 | M | 0.2 | M | NI | 46,XX[3] | |
| 15 | 4, 9 | Common | 98 | 0.03 | M | NI | NI | 20-40,X,add(11)(p11),inc[cp2]/46,XX[11] | ||
| 17 | 6, 1 | T | 96 | 0.03 | M | 0.02 | M | 0.95 | 46,X,t(X;1)(p22;p32),t(4;17)(p16;q?21),del(6)(q?23), del(9)(p?22),del(17)(p12)[cp8]/46,XX[2] | |
| 53 | 6, 4 | Common | 93 | 0.44 | M | 0.02 | M | 0.04 | M | 50-56[5], poor quality |
| 24 | 2, 10 | Common | >98 | 0 | P | 0.09 | P | 0.1 | P | 46,XX[9] |
| Case no. . | Age at diagnosis (y, mo) . | ALL type . | % BM blasts . | D9S1679 . | D9S746 . | D9S761 . | Karyotype . | |||
|---|---|---|---|---|---|---|---|---|---|---|
| Ratio . | POL . | Ratio . | POL . | Ratio . | POL . | |||||
| 22 | 2, 4 | Pre-B | >99 | 0.17 | M | 0.06 | M | 0.02 | NI | 56-57,XXY,[cp3]/46,XY[9] |
| 20 | 3, 7 | Pre-B | 95 | 0.27 | M | 0.05 | M | 0.02 | NI | 46,XX[2] |
| 29 | 3, 1 | Common | >90 | 0.13 | M | 0.84 | 0.81 | 46,XY[20] | ||
| 12 | 4, 7 | Common | 95 | 0.02 | ni | 0.08 | M | 0 | M | 53-59,XXXX,+6,+21,inc[cp2]/46,XX[8] |
| 16 | 4, 7 | Common | 95 | NI | 0.1 | M | 0.13 | M | 45,XX,der(9)t(9;21)(p11;q11.1)t(9;22)(q34;q11),-21[12]/ 46,XX[3] | |
| 21 | 1, 7 | Common | 81-96 | 0.1 | M | 0.2 | M | NI | 46,XX[3] | |
| 15 | 4, 9 | Common | 98 | 0.03 | M | NI | NI | 20-40,X,add(11)(p11),inc[cp2]/46,XX[11] | ||
| 17 | 6, 1 | T | 96 | 0.03 | M | 0.02 | M | 0.95 | 46,X,t(X;1)(p22;p32),t(4;17)(p16;q?21),del(6)(q?23), del(9)(p?22),del(17)(p12)[cp8]/46,XX[2] | |
| 53 | 6, 4 | Common | 93 | 0.44 | M | 0.02 | M | 0.04 | M | 50-56[5], poor quality |
| 24 | 2, 10 | Common | >98 | 0 | P | 0.09 | P | 0.1 | P | 46,XX[9] |
The allele ratios were calculated by using RFLPscan PLUS 3.0 and were normalized against paired remission samples, after electrophoresis on a LI-COR DNA sequencer. BM, bone marrow; POL, parental origin of loss; M, maternal; P, paternal; NI, not informative; T, T-cell lineage; Pre-B, cIg+μB lineage.