Formation of sheep erythrocyte rosettes and cell aggregates of HMC-1 cell subsets
| . | CD2+HMC-1 . | CD2+HMC-1 + T11-1 mAb . | CD2−HMC-1 . | P . |
|---|---|---|---|---|
| Sheep erythrocyte rosettes/HPF | 4.1 ± 1.1 | 0.8 ± 0.5 | 1.2 ± 0.8 | < .05 |
| Aggregates/HPF | 13.2 ± 1.6 | 9.2 ± 2.3 | 10.1 ± 3.8 | .053 |
| Sheep erythrocyte rosettes/event | 4.2 ± 1.0 | 0.7 ± 0.4 | 1.0 ± 0.6 | < .05 |
| Aggregates/event | 13.6 ± 2.7 | 8.4 ± 1.3 | 8.6 ± 1.9 | < .05 |
| . | CD2+HMC-1 . | CD2+HMC-1 + T11-1 mAb . | CD2−HMC-1 . | P . |
|---|---|---|---|---|
| Sheep erythrocyte rosettes/HPF | 4.1 ± 1.1 | 0.8 ± 0.5 | 1.2 ± 0.8 | < .05 |
| Aggregates/HPF | 13.2 ± 1.6 | 9.2 ± 2.3 | 10.1 ± 3.8 | .053 |
| Sheep erythrocyte rosettes/event | 4.2 ± 1.0 | 0.7 ± 0.4 | 1.0 ± 0.6 | < .05 |
| Aggregates/event | 13.6 ± 2.7 | 8.4 ± 1.3 | 8.6 ± 1.9 | < .05 |
CD2+ and CD2− HMC-1 cells were analyzed for sheep erythrocyte rosette formation and aggregation as described in the text. Rosettes and aggregates were counted under an inverted microscope. Values are expressed as counts per high-power field (HPF) as well as counts per events (= rosettes + aggregates + single cells). Results represent the mean ± SD of 6 independent experiments. CD2+ HMC-1 cells were found to form significantly more sheep erythrocyte rosettes and aggregates than CD2− HMC-1. Preincubation of CD2+ HMC-1 cells with mAb 39C1.5 (CD2/T11-1) resulted in an almost complete inhibition of sheep erythrocyte rosette formation and a clear reduction of cell aggregation. By contrast, antibodies against T11-2 (6F10.3), T11-3 (VIT13), as well as the isotype control antibodies showed no effects on rosette or aggregate formation in CD2+ HMC-1 cells (not shown). P values were determined by ANOVA.