Expression of CD2 and other surface markers on cultured MC progenitors
| mAb . | Reactive antigen . | Reactivity of mAb with cultured MCs on various days . | ||
|---|---|---|---|---|
| Day 21 . | Day 42 . | Day 63 . | ||
| SK3 | CD4 | − | − | − |
| L17F12 | CD5 | − | − | − |
| 7G3 | CD123/IL-3Rα | − | − | − |
| 9F5 | CD123/IL-3Rα | − | − | − |
| YB5.B8 | CD117/KIT | + | + | + |
| L178 | CD44/Pgp-1 | + | + | + |
| 1C3 | CD58/LFA-3 | + | + | + |
| T11 | CD2/LFA-2 | − | − | − |
| S5.2 | CD2/LFA-2 | − | − | − |
| RPA-2.10 | CD2/LFA-2 | − | − | − |
| 6F10.3 | CD2/LFA-2 | − | − | − |
| 39C1.5 | CD2/LFA-2 | − | − | − |
| VIT13 | CD2R/LFA-2 | − | − | − |
| mAb . | Reactive antigen . | Reactivity of mAb with cultured MCs on various days . | ||
|---|---|---|---|---|
| Day 21 . | Day 42 . | Day 63 . | ||
| SK3 | CD4 | − | − | − |
| L17F12 | CD5 | − | − | − |
| 7G3 | CD123/IL-3Rα | − | − | − |
| 9F5 | CD123/IL-3Rα | − | − | − |
| YB5.B8 | CD117/KIT | + | + | + |
| L178 | CD44/Pgp-1 | + | + | + |
| 1C3 | CD58/LFA-3 | + | + | + |
| T11 | CD2/LFA-2 | − | − | − |
| S5.2 | CD2/LFA-2 | − | − | − |
| RPA-2.10 | CD2/LFA-2 | − | − | − |
| 6F10.3 | CD2/LFA-2 | − | − | − |
| 39C1.5 | CD2/LFA-2 | − | − | − |
| VIT13 | CD2R/LFA-2 | − | − | − |
Cord blood CD34+ MNCs were cultured as described in the text. After 21, 42, and 63 days, cultured MCs were examined for surface marker expression by flow cytometry. Antibodies against various cytokine receptors and CD2/LFA-2 were applied. Identical results were obtained with MCs cultured in both culture conditions: SCF+IL-6, SCF+IL-4+IL-6+IL-10.