Graft composition and outcome of DLA-haploidentical marrow transplants following 9.2 Gy TBI
| Recipient dog . | Donor dog . | CTL type . | Recipient weight, kg . | Donor graft cell dose . | Time after transplantation . | Findings at necropsy . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| TNC/kg (×108) . | CD34+/kg (×106) . | CD3+/kg (×107) . | CTL/kg (×107) . | Day to ANC > 500/μL . | Day euthanized . | Pathology . | Hematopoietic chimerism . | ||||
| Marrow plus ex vivo expanded CTLs3-150 | |||||||||||
| E468 | E469 | Unmodified | 12.3 | 4.9 | nd | nd | 1.6 | 6 | 10 | GVHD | Donor |
| E466 | E467 | Unmodified | 11.7 | 4.0 | nd | nd | 2.7 | 7 | 10 | GVHD | Donor |
| E473 | E472 | Unmodified | 11.3 | 4.0 | nd | nd | 2.3 | 8 | 11 | GVHD | Donor |
| E646 | E651 | NGFR | 8.5 | 5.0 | 4.5 | 1.7 | 1.5 | 8 | 17 | GVHD | Donor |
| E644 | E642 | GFP | 7.2 | 6.6 | 6.6 | 4.5 | 4.4 | 8 | 19 | GVHD | Donor |
| E650 | E659 | GFP | 10.7 | 3.5 | 4 | 1.7 | 1.1 | 9 | 13 | GVHD | Donor |
| E714 | E715 | GFP | 8.4 | 3.5 | 4.2 | 3.1 | 1.6 | 7 | 10 | GVHD | Donor |
| E671 | E670 | GFP | 13.3 | 6.4 | 5.2 | 3.5 | 1.5 | 6 | 10 | GVHD | Donor |
| E746 | E748 | GFP | 9.2 | 6.4 | 6.5 | 4.8 | 3 | 7 | 12 | GVHD | Donor |
| E716 | E718 | GFP | 11.4 | 3.0 | 3.9 | 1.4 | 1.5 | 7 | 14 | Rejection | Mixed |
| E815 | E817 | GFP | 8.0 | 6.1 | 4 | 1.9 | 2.5 | 8 | 13 | Rejection | Host |
| Marrow only | |||||||||||
| E663 | E661 | n/a | 11.4 | 2.2 | 4.0 | 1.2 | n/a | 9 | 16 | GVHD | Donor |
| E686 | E682 | n/a | 15.0 | 2.5 | 2.8 | 2.9 | n/a | n/a | 13 | Rejection | Host |
| E696 | E693 | n/a | 12.7 | 3.1 | 4.9 | 1.5 | n/a | n/a | 13 | Rejection | Host |
| E691 | E690 | n/a | 11.0 | 2.2 | 2.6 | 1.3 | n/a | 10 | 14 | GVHD | Donor |
| E697 | E696 | n/a | 10.0 | 2.6 | 2.0 | 1.2 | n/a | n/a | 19 | Rejection | Host |
| E024 | E023 | n/a | 9.0 | 4.0 | nd | nd | n/a | n/a | 21 | Rejection | nd |
| E023 | E024 | n/a | 6.5 | 1.0 | nd | nd | n/a | 11 | 25 | GVHD | nd |
| E029 | E030 | n/a | 9.4 | 4.3 | nd | nd | n/a | 8 | 14 | GVHD | nd |
| E030 | E029 | n/a | 12.5 | 3.5 | nd | nd | n/a | n/a | 16 | Rejection | nd |
| E084 | E088 | n/a | 9.8 | 4.1 | nd | nd | n/a | n/a | 15 | Rejection | nd |
| E088 | E084 | n/a | 10.1 | 4.4 | nd | nd | n/a | n/a | 15 | Rejection | nd |
| Recipient dog . | Donor dog . | CTL type . | Recipient weight, kg . | Donor graft cell dose . | Time after transplantation . | Findings at necropsy . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| TNC/kg (×108) . | CD34+/kg (×106) . | CD3+/kg (×107) . | CTL/kg (×107) . | Day to ANC > 500/μL . | Day euthanized . | Pathology . | Hematopoietic chimerism . | ||||
| Marrow plus ex vivo expanded CTLs3-150 | |||||||||||
| E468 | E469 | Unmodified | 12.3 | 4.9 | nd | nd | 1.6 | 6 | 10 | GVHD | Donor |
| E466 | E467 | Unmodified | 11.7 | 4.0 | nd | nd | 2.7 | 7 | 10 | GVHD | Donor |
| E473 | E472 | Unmodified | 11.3 | 4.0 | nd | nd | 2.3 | 8 | 11 | GVHD | Donor |
| E646 | E651 | NGFR | 8.5 | 5.0 | 4.5 | 1.7 | 1.5 | 8 | 17 | GVHD | Donor |
| E644 | E642 | GFP | 7.2 | 6.6 | 6.6 | 4.5 | 4.4 | 8 | 19 | GVHD | Donor |
| E650 | E659 | GFP | 10.7 | 3.5 | 4 | 1.7 | 1.1 | 9 | 13 | GVHD | Donor |
| E714 | E715 | GFP | 8.4 | 3.5 | 4.2 | 3.1 | 1.6 | 7 | 10 | GVHD | Donor |
| E671 | E670 | GFP | 13.3 | 6.4 | 5.2 | 3.5 | 1.5 | 6 | 10 | GVHD | Donor |
| E746 | E748 | GFP | 9.2 | 6.4 | 6.5 | 4.8 | 3 | 7 | 12 | GVHD | Donor |
| E716 | E718 | GFP | 11.4 | 3.0 | 3.9 | 1.4 | 1.5 | 7 | 14 | Rejection | Mixed |
| E815 | E817 | GFP | 8.0 | 6.1 | 4 | 1.9 | 2.5 | 8 | 13 | Rejection | Host |
| Marrow only | |||||||||||
| E663 | E661 | n/a | 11.4 | 2.2 | 4.0 | 1.2 | n/a | 9 | 16 | GVHD | Donor |
| E686 | E682 | n/a | 15.0 | 2.5 | 2.8 | 2.9 | n/a | n/a | 13 | Rejection | Host |
| E696 | E693 | n/a | 12.7 | 3.1 | 4.9 | 1.5 | n/a | n/a | 13 | Rejection | Host |
| E691 | E690 | n/a | 11.0 | 2.2 | 2.6 | 1.3 | n/a | 10 | 14 | GVHD | Donor |
| E697 | E696 | n/a | 10.0 | 2.6 | 2.0 | 1.2 | n/a | n/a | 19 | Rejection | Host |
| E024 | E023 | n/a | 9.0 | 4.0 | nd | nd | n/a | n/a | 21 | Rejection | nd |
| E023 | E024 | n/a | 6.5 | 1.0 | nd | nd | n/a | 11 | 25 | GVHD | nd |
| E029 | E030 | n/a | 9.4 | 4.3 | nd | nd | n/a | 8 | 14 | GVHD | nd |
| E030 | E029 | n/a | 12.5 | 3.5 | nd | nd | n/a | n/a | 16 | Rejection | nd |
| E084 | E088 | n/a | 9.8 | 4.1 | nd | nd | n/a | n/a | 15 | Rejection | nd |
| E088 | E084 | n/a | 10.1 | 4.4 | nd | nd | n/a | n/a | 15 | Rejection | nd |
Abbreviations are explained in the text or Table 2.
Dog E655 (not listed in table) received allogeneic CTLs and marrow but died on day +5. Necropsy showed radiation toxicity with marrow aplasia. This dog was not evaluable for engraftment and was not included in further statistical analysis.