Graft composition and outcome of DLA-haploidentical CD34+ selected PBSC transplants following 9.2 Gy TBI
| Recipient dog . | Donor dog . | CTL type . | Recipient wt, kg . | Donor graft cell dose . | Time after transplantation . | Findings at necropsy . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| TNC/kg (×106) . | CD34+/kg (×106) . | CD3+/kg (×104) . | CTL/kg (×107) . | Day to ANC > 500/μL . | Day euthanized . | Pathology . | Hematopoietic chimerism . | ||||
| CD34+ plus ex vivo expanded CTLs | |||||||||||
| E332 | E330 | Unmodified | 14.4 | 5.0 | 4.0 | <4.0 | 4.0 | n/a | 28 | Rejection | Host |
| E506 | E507 | Unmodified | 10.5 | 1.4 | 1.0 | <1.0 | 2.9 | n/a | 8 | Rejection | Host |
| E554 | E553 | Unmodified | 13.5 | 3.3 | 2.6 | <3.0 | 1.7 | n/a | 14 | Rejection | Host |
| E639* | E607 | Unmodified | 11.7 | 4.0 | 2.2 | <1.0 | 2.0 | 39 | 59 | GVHD* | Donor |
| E538 | E536 | Unmodified | 10.0 | 2.5 | 1.7 | <3.0 | 2.8 | n/a | 21 | Rejection | Host |
| CD34+only | |||||||||||
| E294 | E295 | n/a | 9.9 | 6.0 | 4.2 | <5.0 | n/a | n/a | 21 | Rejection | nd |
| E299 | E300 | n/a | 9.7 | 5.1 | 4.0 | <5.0 | n/a | n/a | 22 | Rejection | nd |
| E511 | E514 | n/a | 10.6 | 7.9 | 3.1 | <8.0 | n/a | n/a | 19 | Rejection | nd |
| E552 | E545 | n/a | 10.3 | 9.8 | 3.0 | <9.0 | n/a | n/a | 27 | Rejection | nd |
| E581 | E577 | n/a | 14.5 | 6.6 | 3.3 | <6.0 | n/a | n/a | 18 | Rejection | nd |
| Recipient dog . | Donor dog . | CTL type . | Recipient wt, kg . | Donor graft cell dose . | Time after transplantation . | Findings at necropsy . | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| TNC/kg (×106) . | CD34+/kg (×106) . | CD3+/kg (×104) . | CTL/kg (×107) . | Day to ANC > 500/μL . | Day euthanized . | Pathology . | Hematopoietic chimerism . | ||||
| CD34+ plus ex vivo expanded CTLs | |||||||||||
| E332 | E330 | Unmodified | 14.4 | 5.0 | 4.0 | <4.0 | 4.0 | n/a | 28 | Rejection | Host |
| E506 | E507 | Unmodified | 10.5 | 1.4 | 1.0 | <1.0 | 2.9 | n/a | 8 | Rejection | Host |
| E554 | E553 | Unmodified | 13.5 | 3.3 | 2.6 | <3.0 | 1.7 | n/a | 14 | Rejection | Host |
| E639* | E607 | Unmodified | 11.7 | 4.0 | 2.2 | <1.0 | 2.0 | 39 | 59 | GVHD* | Donor |
| E538 | E536 | Unmodified | 10.0 | 2.5 | 1.7 | <3.0 | 2.8 | n/a | 21 | Rejection | Host |
| CD34+only | |||||||||||
| E294 | E295 | n/a | 9.9 | 6.0 | 4.2 | <5.0 | n/a | n/a | 21 | Rejection | nd |
| E299 | E300 | n/a | 9.7 | 5.1 | 4.0 | <5.0 | n/a | n/a | 22 | Rejection | nd |
| E511 | E514 | n/a | 10.6 | 7.9 | 3.1 | <8.0 | n/a | n/a | 19 | Rejection | nd |
| E552 | E545 | n/a | 10.3 | 9.8 | 3.0 | <9.0 | n/a | n/a | 27 | Rejection | nd |
| E581 | E577 | n/a | 14.5 | 6.6 | 3.3 | <6.0 | n/a | n/a | 18 | Rejection | nd |
Gy indicates unit of irradiation (1 Gray = 100 rad); TNC/kg, total nucleated cells derived from PBSCs per recipient body weight in kilograms; ANC, absolute neutrophil count; GVHD, graft-versus-host disease determined by histopathologic evaluation; nd, not determined; and n/a, not applicable.
Dog E639 developed clinical manifestations of acute GVHD on day 53 after transplantation with fever, progressive skin erythema, jaundice, anorexia, diarrhea, and weight loss. The dog was euthanized due to worsening condition. Histopathology revealed severe GVHD of the liver and gut, moderate skin GVHD.