Table 1.

Phenotype of retrovirally transduced CTLs

Recipient dogDonor dogPercent specific lysisPercent CD3+Percent CD8+Percent CD4+Percent transducedVector
E646 E651 48 98.2 91.8 19.4 14.1* NGFR 
E644 E642 69 97.7 96.7 5.5 97.7 GFP 
E650 E659 44 99.3 88.7 12.1 96.1 GFP 
E655 E640 57 97.5 95.5 12.4 94.4 GFP 
E714 E715 82 97.1 97.1 13.5 94.1 GFP 
E671 E670 70 98.4 99.1 6.2 93.1 GFP 
E746 E748 68 99.4 96.6 7.8 84.2 GFP 
E716 E718 71 98.3 99.7 7.7 93.4 GFP 
E815 E817 80 99.9 99.8 11.8 98.3 GFP 
E801 E803 74 99.9 99.4 18.7 98.4 GFP 
E755 E753 52 99.9 97.2 88.7 98.8 GFP 
Recipient dogDonor dogPercent specific lysisPercent CD3+Percent CD8+Percent CD4+Percent transducedVector
E646 E651 48 98.2 91.8 19.4 14.1* NGFR 
E644 E642 69 97.7 96.7 5.5 97.7 GFP 
E650 E659 44 99.3 88.7 12.1 96.1 GFP 
E655 E640 57 97.5 95.5 12.4 94.4 GFP 
E714 E715 82 97.1 97.1 13.5 94.1 GFP 
E671 E670 70 98.4 99.1 6.2 93.1 GFP 
E746 E748 68 99.4 96.6 7.8 84.2 GFP 
E716 E718 71 98.3 99.7 7.7 93.4 GFP 
E815 E817 80 99.9 99.8 11.8 98.3 GFP 
E801 E803 74 99.9 99.4 18.7 98.4 GFP 
E755 E753 52 99.9 97.2 88.7 98.8 GFP 

Percent specific lysis denotes cytotoxicity by 51Cr release assay at an effector-target ratio of 20:1.

*

Not selected in G418.

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