Phenotype of splenic plasmacytoid, lymphoid, and myeloid murine DCs
| Cell surface marker . | Plasmacytoid DC CD11c+CD11b−CD8α−B220+ . | Lymphoid DC CD11c+CD11b−CD8α+B220− . | Myeloid DC CD11c+CD11b+CD8α−B220− . | |||
|---|---|---|---|---|---|---|
| Day 0 . | Day 1 . | Day 0 . | Day 1 . | Day 0 . | Day 1 . | |
| CD2 | − | − | − | − | − | − |
| CD4 | + | ± | − | − | − | ± |
| CD5 | − | − | − | − | − | − |
| CD8α | − | − | +++ | +++ | − | − |
| CD11a | ++ | ++ | +++ | +++ | +++ | +++ |
| CD11b | − | − | − | − | +++ | +++ |
| CD11c | + | + | +++ | +++ | +++ | +++ |
| CD19 | − | − | − | − | − | − |
| CD40 | + | ++ | ++ | +++ | + | ++ |
| CD54 | +++ | ++ | +++ | +++ | +++ | +++ |
| CD62L | ++ | − | ++ | − | ± | − |
| CD86 | + | ++ | ++ | ++ | +++ | +++ |
| CD95 (Fas) | ± | ± | ++ | ND | ND | ND |
| B220 (CD45R) | ++ | ++ | − | ± | − | ± |
| Thy1.2 | ++ | ++ | − | − | − | − |
| Gr-1 | ± | + | − | − | − | + |
| H-2Kd (MHC class I) | ++ | ++ | +++ | +++ | +++ | +++ |
| I-Ad(MHC class II) | + | ++ | ++ | +++ | +++ | +++ |
| IL-3R | + | ± | − | − | ± | ± |
| Cell surface marker . | Plasmacytoid DC CD11c+CD11b−CD8α−B220+ . | Lymphoid DC CD11c+CD11b−CD8α+B220− . | Myeloid DC CD11c+CD11b+CD8α−B220− . | |||
|---|---|---|---|---|---|---|
| Day 0 . | Day 1 . | Day 0 . | Day 1 . | Day 0 . | Day 1 . | |
| CD2 | − | − | − | − | − | − |
| CD4 | + | ± | − | − | − | ± |
| CD5 | − | − | − | − | − | − |
| CD8α | − | − | +++ | +++ | − | − |
| CD11a | ++ | ++ | +++ | +++ | +++ | +++ |
| CD11b | − | − | − | − | +++ | +++ |
| CD11c | + | + | +++ | +++ | +++ | +++ |
| CD19 | − | − | − | − | − | − |
| CD40 | + | ++ | ++ | +++ | + | ++ |
| CD54 | +++ | ++ | +++ | +++ | +++ | +++ |
| CD62L | ++ | − | ++ | − | ± | − |
| CD86 | + | ++ | ++ | ++ | +++ | +++ |
| CD95 (Fas) | ± | ± | ++ | ND | ND | ND |
| B220 (CD45R) | ++ | ++ | − | ± | − | ± |
| Thy1.2 | ++ | ++ | − | − | − | − |
| Gr-1 | ± | + | − | − | − | + |
| H-2Kd (MHC class I) | ++ | ++ | +++ | +++ | +++ | +++ |
| I-Ad(MHC class II) | + | ++ | ++ | +++ | +++ | +++ |
| IL-3R | + | ± | − | − | ± | ± |
Expression ranges from ± (low) to +++ (strong). Lymphoid DCs are characterized by a CD11c+CD11b−CD8α+ phenotype, and myeloid DCs exhibit a CD11c+CD11b+CD8α− phenotype. Cells were analyzed directly after FACS sorting or after overnight culture in complete medium.
ND indicates not determined.