Table 1. Primer sequences for RT-PCR and... | American Society of Hematology

Table 1.

Primer sequences for RT-PCR and bisulfite genomic sequencing

	Forward	Reverse	Product size
RT-PCR
ρ-globin	GCTCTGAGTGCTCCCACAGCCGC	CACACTGTGTCCTGCTCTGGGAGC	508
β_A-globin	CACGGGAGCAAGAGCCCAGACCTC	GTTGTAGGTGCTCCGTGATCTTTG	540
β-actin	CGCTCGTTGTTGACAATGGCTC	CCAGTTGGTGACAATACCGTGTTC	216
Bisulfite PCR, top strand
Promoter	TGTAGGGGTGTTTTGTGTAAG	CTATAAAAACACTCAAAACTTAAAAC	263
Exon 1	GGGGTGGGGAGGAGTTGTTAG	AAATTATCAAAAAACCTCTAAATCCA	348
Exon 3	AGAAAGTAGGTGGGAGATAATGTAAGG	TTTTATTAACTTTCACACTATATCCTACTC	358
Bisulfite PCR, bottom strand
Promoter	GTTGTGGGAGTATTTAGAGTTTGGAG	CTTCTACAAAAATACTCTATACAAAAC	271
Exon 1	GTTATTAAAGAATTTTTGGGTTTAGGG	CTCCAAACTCTAAATACTCCCACAAC	291
Exon 3	ATATTGTGTTTTGTTTTGGGAGTTTAGTGG	AAACAAAAAATACTCCACACCTTACTTACC	207

	Forward	Reverse	Product size
RT-PCR
ρ-globin	GCTCTGAGTGCTCCCACAGCCGC	CACACTGTGTCCTGCTCTGGGAGC	508
β_A-globin	CACGGGAGCAAGAGCCCAGACCTC	GTTGTAGGTGCTCCGTGATCTTTG	540
β-actin	CGCTCGTTGTTGACAATGGCTC	CCAGTTGGTGACAATACCGTGTTC	216
Bisulfite PCR, top strand
Promoter	TGTAGGGGTGTTTTGTGTAAG	CTATAAAAACACTCAAAACTTAAAAC	263
Exon 1	GGGGTGGGGAGGAGTTGTTAG	AAATTATCAAAAAACCTCTAAATCCA	348
Exon 3	AGAAAGTAGGTGGGAGATAATGTAAGG	TTTTATTAACTTTCACACTATATCCTACTC	358
Bisulfite PCR, bottom strand
Promoter	GTTGTGGGAGTATTTAGAGTTTGGAG	CTTCTACAAAAATACTCTATACAAAAC	271
Exon 1	GTTATTAAAGAATTTTTGGGTTTAGGG	CTCCAAACTCTAAATACTCCCACAAC	291
Exon 3	ATATTGTGTTTTGTTTTGGGAGTTTAGTGG	AAACAAAAAATACTCCACACCTTACTTACC	207

RT-PCR indicates reverse transcriptase–polymerase chain reaction.