The effects of varying concentrations of murine and human MIP-1α on CFU-A colony formation in vitro
| Chemokine, ng/mL . | Colony growth (%)* . | Receptors used . |
|---|---|---|
| muMIP-1α (CCL3) | ||
| 5 | 51.3 ± 32.0 | CCR1, CCR3, CCR5, D6 |
| 12.5 | 61.5 ± 15.4 | |
| 25 | 20.5 ± 17.8 | |
| 50 | 0.0 ± 0.0 | |
| MIP-1αP | ||
| 5 | 118.9 ± 27.1 | CCR1, CCR3, CCR5, D6 |
| 10 | 86.8 ± 13.5 | |
| 30 | 51.3 ± 16.3 | |
| 50 | 51.3 ± 24.8 | |
| 75 | 32.9 ± 4.7 | |
| MIP-1αS | ||
| 5 | 71.0 ± 25.2 | CCR1 |
| 10 | 71.0 ± 7.2 | |
| 30 | 35.5 ± 3.6 | |
| 50 | 35.5 ± 12.0 | |
| 75 | 30.3 ± 10.0 |
| Chemokine, ng/mL . | Colony growth (%)* . | Receptors used . |
|---|---|---|
| muMIP-1α (CCL3) | ||
| 5 | 51.3 ± 32.0 | CCR1, CCR3, CCR5, D6 |
| 12.5 | 61.5 ± 15.4 | |
| 25 | 20.5 ± 17.8 | |
| 50 | 0.0 ± 0.0 | |
| MIP-1αP | ||
| 5 | 118.9 ± 27.1 | CCR1, CCR3, CCR5, D6 |
| 10 | 86.8 ± 13.5 | |
| 30 | 51.3 ± 16.3 | |
| 50 | 51.3 ± 24.8 | |
| 75 | 32.9 ± 4.7 | |
| MIP-1αS | ||
| 5 | 71.0 ± 25.2 | CCR1 |
| 10 | 71.0 ± 7.2 | |
| 30 | 35.5 ± 3.6 | |
| 50 | 35.5 ± 12.0 | |
| 75 | 30.3 ± 10.0 |
Results are expressed as the mean percentage (± SD) of colonies generated in the presence of chemokine compared with control plates. Results are representative of at least 5 experiments. MIP indicates macrophage inflammatory protein; CFU-A, colony-forming unit–agar.